{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T12:54:27Z","timestamp":1793451267708,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,9,16]],"date-time":"2017-09-16T00:00:00Z","timestamp":1505520000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["NIH\/1R01HL105764"],"award-info":[{"award-number":["NIH\/1R01HL105764"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006827","name":"Florida Department of Health","doi-asserted-by":"publisher","award":["7JK05"],"award-info":[{"award-number":["7JK05"]}],"id":[{"id":"10.13039\/100006827","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670429"],"award-info":[{"award-number":["81670429"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>Myh11\u2010CreER<jats:sup>T2<\/jats:sup><\/jats:italic> mouse line (<jats:italic>Cre<jats:sup>+<\/jats:sup><\/jats:italic>) has gained increasing application because of its high lineage specificity relative to other Cre drivers targeting smooth muscle cells (SMCs). This <jats:italic>Cre<\/jats:italic> allele, however, was initially inserted into the Y chromosome (X\/Y<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup>), which excluded its application in female mice. Our group established a <jats:italic>Cre<jats:sup>+<\/jats:sup><\/jats:italic> colony from male ancestors. Surprisingly, genotype screening identified female carriers that stably transmitted the <jats:italic>Cre<\/jats:italic> allele to the following generations. Crossbreeding experiments revealed a pattern of X\u2010linked inheritance for the transgene (<jats:italic>k<\/jats:italic>\u2009&gt;\u20091000), indicating that these female carries acquired the <jats:italic>Cre<\/jats:italic> allele through a mechanism of Y to X chromosome translocation. Further characterization demonstrated that in hemizygous X\/X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup> mice Cre activity was restricted to a subset arterial SMCs, with Cre expression in arteries decreased by 50% compared to X\/Y<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup> mice. This mosaicism, however, diminished in homozygous X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup>\/X<jats:italic><jats:sup>Cre+<\/jats:sup><\/jats:italic> mice. In a model of aortic aneurysm induced by a SMC\u2010specific <jats:italic>Tgfbr1<\/jats:italic> deletion, the homozygous X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup>\/X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup> Cre driver unmasked the aortic phenotype that is otherwise subclinical when driven by the hemizygous X\/X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup> Cre line. In conclusion, the <jats:italic>Cre<\/jats:italic> allele carried by this female mouse line is located on the X chromosome and subjected to X\u2010inactivation. The homozygous X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup>\/X<jats:sup><jats:italic>Cre+<\/jats:italic><\/jats:sup> mice produce uniform Cre activity in arterial SMCs.<\/jats:p>","DOI":"10.1002\/dvg.23054","type":"journal-article","created":{"date-parts":[[2017,8,28]],"date-time":"2017-08-28T07:16:56Z","timestamp":1503904616000},"source":"Crossref","is-referenced-by-count":24,"title":["An X\u2010linked <i>Myh11\u2010CreER<sup>T2<\/sup><\/i> mouse line resulting from Y to X chromosome\u2010translocation of the <i>Cre<\/i> allele"],"prefix":"10.1002","volume":"55","author":[{"given":"Mingmei","family":"Liao","sequence":"first","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"},{"name":"Department of Surgery Xiangya Hospital Central South University Changsha Peoples Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junmei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"},{"name":"Institute of Cardiovascular Disease, University of South China Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin H.","family":"Ali","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin L.","family":"Chan","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of Florida College of Public Health &amp; Health Professions College of Medicine Gainesville Florida 32611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Offermanns","sequence":"additional","affiliation":[{"name":"Max\u2010Planck\u2010Institute for Heart and Lung Research, University of Heidelberg Bad Nauheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhisheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease, University of South China Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8057-823X","authenticated-orcid":false,"given":"Zhihua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine Gainesville Florida 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhr214"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3366"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-011-1011-z"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000100554"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-471-1_18"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.06.067"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.255786"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs115"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.1016"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000249623.79871.bc"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.115.306573"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200009)28:1<15::AID-GENE20>3.0.CO;2-C"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.132.5.849"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI69942"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-15393"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.968792"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.281048"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2003"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.5.363"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-007-9134-7"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00521.2014"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3866"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.05.041"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20448"},{"key":"e_1_2_6_26_1","first-page":"454","article-title":"A pronounced evolutionary shift of the pseudoautosomal region boundary in house mice. 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