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The mechanisms underlying these events remain largely unknown due to a lack of experimental models for identifying perivascular astrocytes and distinguishing these cell types from other astroglial populations. Megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1) is a transmembrane protein that is expressed in perivascular astrocyte end feet where it controls BBB development and homeostasis. On the basis of this knowledge, we used T2A peptide\u2010skipping strategies to engineer a knock\u2010in mouse model in which the endogenous <jats:italic>Mlc1<\/jats:italic> gene drives expression of enhanced green fluorescent protein (eGFP), without impacting expression of Mlc1 protein. Analysis of fetal, neonatal and adult Mlc1\u2010eGFP knock\u2010in mice revealed a dynamic spatiotemporal expression pattern of eGFP in glial cells, including nestin\u2010expressing neuroepithelial cells during development and glial fibrillary acidic protein (GFAP)\u2010expressing perivascular astrocytes in the postnatal brain. EGFP was not expressed in neurons, microglia, oligodendroglia, or cerebral vascular cells. Analysis of angiogenesis in the neonatal retina also revealed enriched Mlc1\u2010driven eGFP expression in perivascular astrocytes that contact sprouting blood vessels and regulate blood\u2010retinal barrier permeability. A cortical injury model revealed that Mlc1\u2010eGFP expression is progressively induced in reactive astrocytes that form a glial scar. Hence, Mlc1\u2010eGFP knock\u2010in mice are a new and powerful tool to identify perivascular astrocytes in the brain and retina and characterize how these cell types regulate cerebral blood vessel functions in health and disease.<\/jats:p>","DOI":"10.1002\/dvg.23071","type":"journal-article","created":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T08:48:46Z","timestamp":1505897326000},"source":"Crossref","is-referenced-by-count":14,"title":["Selective expression of eGFP in mouse perivascular astrocytes by modification of the <i>Mlc1<\/i> gene using T2A\u2010based ribosome skipping"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3075-7680","authenticated-orcid":false,"given":"Jordan J.","family":"Toutounchian","sequence":"first","affiliation":[{"name":"Department of Neurosurgery The University of Texas M. D. Anderson Cancer Center Houston Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph H.","family":"McCarty","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The University of Texas M. D. 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