{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:48:25Z","timestamp":1753868905070,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1EY026614"],"award-info":[{"award-number":["RO1EY026614"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>LHX3, a LIM\u2010homeodomain transcription factor, is broadly expressed in the developing pituitary, spinal cord, medulla, retina and inner ear, and plays essential roles during embryonic development. Mice with homozygous <jats:italic>Lhx3<\/jats:italic> null mutation exhibit failure in the formation of pituitary gland and die perinatally. To facilitate the functional study of <jats:italic>Lhx3<\/jats:italic> in mice, we engineered and characterized two novel <jats:italic>Lhx3<\/jats:italic> mouse strains: <jats:italic>Lhx3<jats:sup>GFP<\/jats:sup><\/jats:italic> reporter knock\u2010in and <jats:italic>Lhx3<jats:sup>loxP<\/jats:sup><\/jats:italic> conditional knockout mice. Coimmunolabeling of LHX3 and GFP shows that the expression pattern of the knock\u2010in GFP reporter recapitulates that of endogenous LHX3 in cochlea, vestibule, retina, and spinal cord. By crossing <jats:italic>Lhx3<jats:sup>loxP<\/jats:sup><\/jats:italic> mice with the ubiquitous <jats:italic>CMV\u2010Cre<\/jats:italic> mice, we have demonstrated a high efficiency of Cre recombinase\u2010mediated removal of exons 3 to 5 of <jats:italic>Lhx3<\/jats:italic>, which encode the second LIM\u2010domain and the HD domain of LHX3, resulting global knockout of <jats:italic>Lhx3<\/jats:italic>. Thus, <jats:italic>Lhx3<jats:sup>GFP<\/jats:sup><\/jats:italic> and <jats:italic>Lhx3<jats:sup>loxP<\/jats:sup><\/jats:italic> mice serve as valuable genetic tools to dissect the tissue\u2010specific roles of <jats:italic>Lhx3<\/jats:italic> at late\u2010gestation and postnatal stages in mice.<\/jats:p>","DOI":"10.1002\/dvg.23098","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T08:06:40Z","timestamp":1520323600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Generation and characterization of <i>Lhx3<sup>GFP<\/sup><\/i> reporter knockin and <i>Lhx3<sup>loxP<\/sup><\/i> conditional knockout mice"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2638-9232","authenticated-orcid":false,"given":"Mei","family":"Xu","sequence":"first","affiliation":[{"name":"Institute of Life Sciences, Hangzhou Normal University Hangzhou Zhejiang 310036 China"},{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester Rochester New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester Rochester New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuhui","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, Hangzhou Normal University Hangzhou Zhejiang 310036 China"},{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester Rochester New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqing","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, Hangzhou Normal University Hangzhou Zhejiang 310036 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester Rochester New York 14642"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2720"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2013.12.001"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","unstructured":"Blixt M. K. E. Konjusha D. Ring H. &Hallbook F.(2017). Zinc finger gene nolz1 regulates the formation of retinal progenitor cells and suppresses the Lim3\/Lhx3 phenotype of retinal bipolar cells in chicken retina.Developmental Dynamics.","DOI":"10.1002\/dvdy.24607"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-011-1393-x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3951-07.2007"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/344876a0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(99)01883-1"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21735"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2007.05.002"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-0325"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn114"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12902-017-0164-8"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.24.5080"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1994.13.1163"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5264.1004"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103888200"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1237"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00823-1"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002020405"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1995.1004"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.23098","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T11:15:00Z","timestamp":1695554100000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,25]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/dvg.23098"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23098","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"type":"print","value":"1526-954X"},{"type":"electronic","value":"1526-968X"}],"subject":[],"published":{"date-parts":[[2018,3,25]]},"assertion":[{"value":"2018-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23098"}}