{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:47:10Z","timestamp":1753868830473,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T00:00:00Z","timestamp":1533859200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007386","name":"Pro-Reitoria de Pesquisa, Universidade de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["109,300\/2007\u20106","116,601\/2008 6"],"award-info":[{"award-number":["109,300\/2007\u20106","116,601\/2008 6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In a previous bioinformatics analysis we identified 10 conserved <jats:italic>Drosophila melanogaster<\/jats:italic> sequences that reside upstream from protein coding genes (<jats:italic>CGs<\/jats:italic>). Here we characterize one of these genomic regions, which constitutes a <jats:italic>Drosophila melanogaster cis<\/jats:italic>\u2010regulatory module (<jats:italic>CRM<\/jats:italic>) that we denominate <jats:italic>TT\u2010CRM<\/jats:italic>. The <jats:italic>TT\u2010CRM<\/jats:italic> is 646\u2009bp long and is located in one of the introns of <jats:italic>CG32239<\/jats:italic> and resides about 3,500\u2009bp upstream of <jats:italic>CG13711<\/jats:italic> and about 620\u2009bp upstream of <jats:italic>CG12493<\/jats:italic>. Analysis of <jats:italic>646\u2009bp\u2010lacZ<\/jats:italic> lines revealed that <jats:italic>TT\u2010CRM<\/jats:italic> drives gene expression not only to the larval, prepupal, and pupal tracheal system but also to the adult dorsal longitudinal muscles. The patterns of mRNA expression of the transgene and of the <jats:italic>CGs<\/jats:italic> that lie in the vicinity of <jats:italic>TT\u2010CRM<\/jats:italic> were investigated both in dissected trachea and in adult thoraces. Through RT\u2010qPCR we observed that in the tracheal system the pattern of expression of <jats:italic>646\u2009bp\u2010lacZ<\/jats:italic> is similar to the pattern of expression of <jats:italic>CG32239<\/jats:italic> and <jats:italic>CG13711<\/jats:italic>, whereas in the thoracic muscles <jats:italic>646\u2009bp\u2010lacZ<\/jats:italic> expression accompanies the expression of <jats:italic>CG12493<\/jats:italic>. Together, these results suggest new functions for two previously characterized <jats:italic>D. melanogaster<\/jats:italic> genes and also contribute to the initial characterization of a novel CRM that drives a dynamic pattern of expression throughout development.<\/jats:p>","DOI":"10.1002\/dvg.23222","type":"journal-article","created":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T17:44:31Z","timestamp":1533923071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of a novel <i>Drosophila melanogaster cis<\/i>\u2010regulatory module that drives gene expression to the larval tracheal system and adult thoracic musculature"],"prefix":"10.1002","volume":"56","author":[{"given":"Jorge Victor Wilfredo Cachay","family":"Wester","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biologia Celular e Molecular, Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Antonio Couto","family":"Lima","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica e Qu\u00edmica, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maiaro Cabral Rosa","family":"Machado","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular e Molecular e Bioagentes Patog\u00eanicos, Faculdade de Medicina de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Vieira","family":"Zampar","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Sakagute","family":"Tavares","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6068-7690","authenticated-orcid":false,"given":"Nadia","family":"Monesi","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.6.2977"},{"volume-title":"Drosophila. A laboratory handbook","year":"1989","author":"Ashburner M.","key":"e_1_2_6_3_1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270050058"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200110077"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20239"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00589-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00795-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22728"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2015.01.014"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072408jd"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.134924"},{"issue":"1","key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1242\/dev.113.1.67","article-title":"Development of the indirect flight muscles of Drosophila","volume":"113","author":"Fernandes J.","year":"1991","journal-title":"Development"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23075"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","unstructured":"Gerbasi V. R. Preall J. B. Golden D. E. Powell D. W. Cummins T. D. &Sontheimer E. J.(2011). Blanks a nuclear siRNA\/dsRNA\u2010binding complex component is required forDrosophilaspermiogenesis.Proceedings of the National Academy of Sciences of the United States of America 108(8) 3204\u20133209.https:\/\/doi.org\/10.1073\/pnas.1009781108","DOI":"10.1073\/pnas.1009781108"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.08.032"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.11.017"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2017.04.005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00334868"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060137"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20364"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90173-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.09.029"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/21541264.2015.1130118"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.04.010"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.2003.00408.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-9112-4_6"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00344233"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(92)90139-6"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056408"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2011.03.014"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genom-091212-153423"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6289436"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.11.010"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00202-2"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10559"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3715005"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02588"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22946"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6289435"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.5.1640"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90177-1"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158712"},{"key":"e_1_2_6_46_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1242\/jcs.s3-98.41.123","article-title":"The post\u2010embryonic development of the tracheal system in Drosophila melanogaster","volume":"98","author":"Whitten J. M.","year":"1957","journal-title":"Journal of Cell Science"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.23222","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T12:44:36Z","timestamp":1694263476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1002\/dvg.23222"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23222","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"type":"print","value":"1526-954X"},{"type":"electronic","value":"1526-968X"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"2018-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23222"}}