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Accordingly, perturbation of PLZF function results in a myriad of physiologic defects, the most conspicuous of which is abnormal skeletal patterning. Although whole body knockout of <jats:italic>Plzf<\/jats:italic> in the mouse (<jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>KO<\/jats:italic><\/jats:sup>) has significantly expanded our understanding of <jats:italic>Plzf<\/jats:italic> function in vivo, a conditional knockout mouse model that enables tissue or cell\u2010type specific ablation of <jats:italic>Plzf<\/jats:italic> has not been developed. Therefore, we used CRISPR\/Cas 9 gene editing to generate a mouse model in which exon 2 of the murine <jats:italic>Plzf<\/jats:italic> gene is specifically flanked (or floxed) by <jats:italic>LoxP<\/jats:italic> sites (<jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>f\/f<\/jats:italic><\/jats:sup>). Crossing our <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>f\/f<\/jats:italic><\/jats:sup> mouse with a global cre\u2010driver mouse to generate the <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>d\/d<\/jats:italic><\/jats:sup> bigenic mouse, we demonstrate that exon 2 of the <jats:italic>Plzf<\/jats:italic> gene is ablated in the <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>d\/d<\/jats:italic><\/jats:sup> bigenic. Similar to the previously reported <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>KO<\/jats:italic><\/jats:sup> mouse, the <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>d\/d<\/jats:italic><\/jats:sup> mouse exhibits a severe defect in skeletal patterning of the hindlimb, indicating that the <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>f\/f<\/jats:italic><\/jats:sup> mouse functions as designed. Therefore, studies in this short technical report demonstrate that the <jats:italic>Plzf<\/jats:italic><jats:sup>\n<jats:italic>f\/f<\/jats:italic><\/jats:sup> mouse will be useful to investigators who wish to explore the role of the Plzf transcription factor in a specific tissue or cell\u2010type.<\/jats:p>","DOI":"10.1002\/dvg.23281","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T02:46:43Z","timestamp":1547088403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Using CRISPR\/Cas9 engineering to generate a mouse with a conditional knockout allele for the promyelocytic leukemia zinc finger transcription factor"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9668-7593","authenticated-orcid":false,"given":"Lan","family":"Hai","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza, Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2715-2752","authenticated-orcid":false,"given":"Maria M.","family":"Szwarc","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza, Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise G.","family":"Lanza","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  One Baylor Plaza, Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason D.","family":"Heaney","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  One Baylor Plaza, Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-636X","authenticated-orcid":false,"given":"John P.","family":"Lydon","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza, Houston 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