{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:03:26Z","timestamp":1791896606327,"version":"4.3.4"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005440","name":"Center for Scientific Review","doi-asserted-by":"publisher","award":["NIH R03 HL140264"],"award-info":[{"award-number":["NIH R03 HL140264"]}],"id":[{"id":"10.13039\/100005440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","award":["Gilead Sciences Research Scholars Program"],"award-info":[{"award-number":["Gilead Sciences Research Scholars Program"]}],"id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03 HL140264"],"award-info":[{"award-number":["R03 HL140264"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Nppa is a cardiac hormone which plays critical roles in regulating salt\u2013water balance. Its expression is restricted to the atria of the healthy post\u2010natal heart. During heart development, spatio\u2010temporal expression of Nppa is dynamically changed within the heart and becomes restricted to the atria upon birth. In contrast to its atrial specific expression after birth, Nppa is re\u2010expressed in the adult ventricles in response to cardiac hypertrophy. To study cardiac chamber specification during development and pathological cardiac remodeling during heart disease, we generated a novel <jats:italic>Nppa<\/jats:italic> reporter mouse line by knocking\u2010in a <jats:italic>tagBFP<\/jats:italic> reporter cassette into 3\u2032\u2010UTR of the Nppa gene without disrupting the endogenous gene. Our results demonstrated dynamic tagBFP expression in the developing heart, recapitulating the spatiotemporal expression pattern of endogenous Nppa. We also found that Nppa\u2010tagBFP is induced in the ventricle during pathological remodeling. Taken together, <jats:italic>Nppa\u2010tagBFP<\/jats:italic> reporter knock\u2010in mouse model described in this article will serve as a valuable tool to study cardiac chamber specification during development as well as pathological cardiac remodeling.<\/jats:p>","DOI":"10.1002\/dvg.23294","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T15:43:19Z","timestamp":1553787799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Generation of <i>Nppa\u2010tagBFP<\/i> reporter knock\u2010in mouse line for studying cardiac chamber specification"],"prefix":"10.1002","volume":"57","author":[{"given":"Zhentao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Cardiovascular Medicine Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Cell and Developmental Biology Vanderbilt University  Nashville Tennessee"},{"name":"Vanderbilt Center for Stem Cell Biology Vanderbilt University  Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinkun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiovascular Medicine Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Pharmacology Vanderbilt University  Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hind","family":"Lal","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiovascular Medicine Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Pharmacology Vanderbilt University  Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3063-2036","authenticated-orcid":false,"given":"Young\u2010Jae","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiovascular Medicine Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Cell and Developmental Biology Vanderbilt University  Nashville Tennessee"},{"name":"Vanderbilt Center for Stem Cell Biology Vanderbilt University  Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90512-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1710"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.15.1835945"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9753"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.227405"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223925"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.222902"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.10.004"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118166"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10220"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.114025"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132292"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1139\/y91-223"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23256"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41407"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23294","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvg.23294","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23294","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T09:44:03Z","timestamp":1694339043000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23294"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,28]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/dvg.23294"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23294","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,28]]},"assertion":[{"value":"2019-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23294"}}