{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T06:58:32Z","timestamp":1776754712885,"version":"3.51.2"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Ophthalmology at the University of Rochester","award":["RO1EY026614"],"award-info":[{"award-number":["RO1EY026614"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>LHX4 is a LIM\u2010homeodomain transcription factor essential for the development of spinal cord and pituitary gland. Mice with homozygous <jats:italic>Lhx4\u2010null<\/jats:italic> mutation suffer early postnatal death from lung defect. In this study, to facilitate the research on <jats:italic>Lhx4<\/jats:italic> function, we designed a targeting construct to generate two novel <jats:italic>Lhx4<\/jats:italic> mouse lines: <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>loxP<\/jats:italic><\/jats:sup> conditional knockout and <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>tdT<\/jats:italic><\/jats:sup> reporter knock\u2010in mice. <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>tdT\/+<\/jats:italic><\/jats:sup>, <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>loxP\/+<\/jats:italic><\/jats:sup>, and <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>loxP\/loxP<\/jats:italic><\/jats:sup> were viable, fertile, and did not display any gross abnormalities. By breeding <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>loxP<\/jats:italic><\/jats:sup> line with Cre\u2010expressing mice, the Exon 3 of <jats:italic>Lhx4<\/jats:italic> was efficiently removed, resulting in a shift in the reading frame and the inactivation of <jats:italic>Lhx4<\/jats:italic>. The expression of tdTomato knock\u2010in reporter recapitulated the endogenous LHX4 expression and was detected in the retina, spinal cord, pituitary gland, and hindbrain of <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>tdT<\/jats:italic><\/jats:sup> mice. Thus, <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>tdT<\/jats:italic><\/jats:sup> and <jats:italic>Lhx4<\/jats:italic><jats:sup>\n<jats:italic>loxP<\/jats:italic><\/jats:sup> mouse lines provide valuable tools for unraveling the tissue\u2010specific role of <jats:italic>Lhx4<\/jats:italic> at postnatal stages in mice.<\/jats:p>","DOI":"10.1002\/dvg.23328","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T09:24:48Z","timestamp":1563355488000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Generation and characterization of <i>Lhx4<\/i><sup><i>tdT<\/i><\/sup> reporter knock\u2010in and <i>Lhx4<\/i><sup><i>loxP<\/i><\/sup> conditional knockout mice"],"prefix":"10.1002","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2015-6868","authenticated-orcid":false,"given":"Xuhui","family":"Dong","sequence":"first","affiliation":[{"name":"College of Life Sciences Zhejiang University  Hangzhou Zhejiang China"},{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"},{"name":"Institute of Life Sciences Hangzhou Normal University  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luming","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Sciences Zhejiang University  Hangzhou Zhejiang China"},{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"},{"name":"Institute of Life Sciences Hangzhou Normal University  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiadong","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Zhejiang University  Hangzhou Zhejiang China"},{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"},{"name":"Institute of Life Sciences Hangzhou Normal University  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqing","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences Hangzhou Normal University  Hangzhou Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5278-4554","authenticated-orcid":false,"given":"Lin","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Flaum Eye Institute University of Rochester  Rochester New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,17]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2013.12.001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.032"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-2389"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3951-07.2007"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<130::AID-GENE9>3.0.CO;2-I"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-4484"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20000814)424:1<1::AID-CNE1>3.0.CO;2-V"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24067"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1505870112"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06582.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/323764"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.24.5080"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81704-3"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5344.1809"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.K06-200"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.4852"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.23328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvg.23328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T23:02:55Z","timestamp":1694214175000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,17]]},"references-count":16,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1002\/dvg.23328"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23328","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,17]]},"assertion":[{"value":"2019-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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