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Given its impact on human society, we aimed in the current study to assess whether sleep deprivation affects blastocyst gene expression and\/or the implantation process. For this, pregnant mice (gestational day 0 [GD 0]) were assigned into paradoxical sleep deprivation (SD, 72\u2009hr; multiple platform method) and, a control (CT) group. Animals were euthanized on GD 3.5 and blood, uterus (embryos) and fallopian tube were collected. Then, 89% of CT presented blastocysts in the uterus versus 25% from <jats:italic>SD<\/jats:italic> group. Compared to CT, <jats:italic>SD<\/jats:italic> presented lighter relative uterus weight, increased plasma concentrations of corticosterone and testosterone, decreased concentrations of progesterone and luteinizing hormone, but no statistical differences in plasma concentrations of 17\u03b2\u2010estradiol and follicle stimulating hormone. There were no differences in uterus and blastocyst gene expression related to embryo implantation and development, and no alteration in blastocysts global DNA methylation. Considering this, the decreased pregnancy maintenance after sleep deprivation seems not to be associated with implantation losses or developmental problems related to the blastocysts. It is likely that complications in morula development and\/or its movement through the fallopian tubes affect the pregnancy rate, since only 25% of SD females presented a blastocyst on the GD 3.5. In fact, three out of four females without blastocysts in the uterus presented morula in the fallopian tubes due to a phase delay. Additionally, we suggest that the observed hormonal changes may play a role in this outcome.<\/jats:p>","DOI":"10.1002\/dvg.23350","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T12:25:16Z","timestamp":1575894316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus"],"prefix":"10.1002","volume":"58","author":[{"given":"Bruno Frederico Aguilar","family":"Calegare","sequence":"first","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Azzolini","sequence":"additional","affiliation":[{"name":"Department of Urology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Ribeiro","family":"da Silva Vallim","sequence":"additional","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson Guimar\u00e3es Lo","family":"Turco","sequence":"additional","affiliation":[{"name":"Department of Urology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila Farias","family":"Tempaku","sequence":"additional","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7729-9985","authenticated-orcid":false,"given":"Vanessa Cavalcante","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Tufik","sequence":"additional","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e2nia","family":"D'Almeida","sequence":"additional","affiliation":[{"name":"Department of Psychobiology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-011-9696-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874306401105010031"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01339.x"},{"key":"e_1_2_7_5_1","first-page":"167","article-title":"Princ\u00edpios eticos e pr\u00e1ticos do uso de animais de experimenta\u00e7\u00e3o","volume":"1","author":"Andersen M. 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