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We have previously generated a constitutively activated <jats:italic>Acvr1<\/jats:italic>(<jats:italic>ca\u2010Acvr1<\/jats:italic>) transgenic mouse line (line L35) through pronuclei injection to investigate impacts of enhanced BMP signaling in a tissue specific manner. However, line L35 shows a restricted expression pattern of the transgene. Here, we generated another <jats:italic>ca\u2010Acvr1<\/jats:italic> transgenic line, line A11, using embryonic stem (ES) transgenesis. The generated line A11 shows distinctive phenotypes from line L35, along with very limited expression levels of the transgene. When the transgene is activated in the neural crest cells in a Cre\u2010dependent manner, line A11 exhibits cleft palate and shorter jaws, while line L35 develops ectopic cartilages and highly hypomorphic facial structures. When activated in limb buds, line A11 develops organized but smaller limb skeletal structures, while line L35 forms disorganized limbs with little mineralization. Additionally, no heterotopic ossification (HO) is identified in line A11 when bred with <jats:italic>NFATc1\u2010Cre<\/jats:italic> mice even after induction of tissue injury, which is an established protocol for HO for line L35. Therefore, the newly generated conditional <jats:italic>ca\u2010Acvr1<\/jats:italic> mouse line A11 provides an additional resource to dissect highly context dependent functions of BMP signaling in development and disease.<\/jats:p>","DOI":"10.1002\/dvg.23419","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T01:09:20Z","timestamp":1618448960000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Generation of a new mouse line with conditionally activated signaling through the <scp>BMP<\/scp> receptor, <scp>ACVR1<\/scp>: A tool to characterize pleiotropic roles of <scp>BMP<\/scp> functions"],"prefix":"10.1002","volume":"59","author":[{"given":"Jingwen","family":"Yang","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology &amp; Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology Wuhan University  Wuhan Hubei 430079 China"},{"name":"Department of Biologic and Materials Sciences &amp; Prosthodontics, School of Dentistry University of Michigan  MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Toda Nakamura","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences &amp; Prosthodontics, School of Dentistry University of Michigan  MI USA"},{"name":"Department of Oral Growth and Development Fukuoka Dental College  Hakata Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn A.","family":"Hallett","sequence":"additional","affiliation":[{"name":"Department of Biologic and Materials Sciences &amp; Prosthodontics, School of Dentistry University of Michigan  MI USA"},{"name":"Department of Orthodontics and Pediatric Dentistry School of Dentistry, University of Michigan  MI 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