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Here, we report on the generation of a transgenic mouse expressing the tamoxifen inducible <jats:italic>CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup> under the control of <jats:italic>Cldn11<\/jats:italic> promoter that allows, for the first time, selective and temporally controlled targeting of lymphatic valve endothelial cells. We show that within the vasculature CLDN11 is specifically expressed in lymphatic valves but is not required for their development as mice with a global loss of <jats:italic>Cldn11<\/jats:italic> display normal valves in the mesentery. Tamoxifen treated <jats:italic>Cldn11\u2010CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup> mice also carrying a fluorescent Cre\u2010reporter displayed reporter protein expression selectively in lymphatic valves and, to a lower degree, in venous valves. Analysis of developing vasculature further showed that <jats:italic>Cldn11\u2010CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup>\u2010mediated recombination is induced during valve leaflet formation, and efficient labeling of valve endothelial cells was observed in mature valves. The <jats:italic>Cldn11<\/jats:italic>\u2010<jats:italic>CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup> mouse thus provides a valuable tool for functional studies of valves.<\/jats:p>","DOI":"10.1002\/dvg.23439","type":"journal-article","created":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T13:57:23Z","timestamp":1627912643000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["An inducible <scp><i>Cldn11\u2010CreER<\/i><sup><i>T2<\/i><\/sup><\/scp> mouse line for selective targeting of lymphatic valves"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4046-4702","authenticated-orcid":false,"given":"Henrik","family":"Orts\u00e4ter","sequence":"first","affiliation":[{"name":"Department of Immunology, Genetics and Pathology Uppsala University  Uppsala 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