{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:10:08Z","timestamp":1784247008832,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,19]],"date-time":"2021-10-19T00:00:00Z","timestamp":1634601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD 084409"],"award-info":[{"award-number":["HD 084409"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE 026434"],"award-info":[{"award-number":["DE 026434"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["OD 010997"],"award-info":[{"award-number":["OD 010997"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["OD 030008"],"award-info":[{"award-number":["OD 030008"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The vertebrate Six (Sine oculis homeobo\n                    <jats:italic>x<\/jats:italic>\n                    ) family of homeodomain transcription factors plays critical roles in the development of several organs. Six1 plays a central role in cranial placode development, including the precursor tissues of the inner ear, as well as other cranial sensory organs and the kidney. In humans, mutations in\n                    <jats:italic>SIX1<\/jats:italic>\n                    underlie some cases of Branchio\u2010oto\u2010renal (BOR) syndrome, which is characterized by moderate\u2010to\u2010severe hearing loss. We utilized CRISPR\/Cas9 technology to establish a\n                    <jats:italic>six1<\/jats:italic>\n                    mutant line in\n                    <jats:italic>Xenopus tropicalis<\/jats:italic>\n                    that is available to the research community. We demonstrate that at larval stages, the\n                    <jats:italic>six1\u2010null<\/jats:italic>\n                    animals show severe disruptions in gene expression of putative Six1 target genes in the otic vesicle, cranial ganglia, branchial arch, and neural tube. At tadpole stages,\n                    <jats:italic>six1\u2010null<\/jats:italic>\n                    animals display dysmorphic Meckel's, ceratohyal, and otic capsule cartilage morphology. This mutant line will be of value for the study of the development of several organs as well as congenital syndromes that involve these tissues.\n                  <\/jats:p>","DOI":"10.1002\/dvg.23453","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T05:32:10Z","timestamp":1634794330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Generation of a new\n                    <i>six1<\/i>\n                    \u2010null line in\n                    <i>Xenopus tropicalis<\/i>\n                    for study of development and congenital disease"],"prefix":"10.1002","volume":"59","author":[{"given":"Kelsey","family":"Coppenrath","sequence":"first","affiliation":[{"name":"Eugene Bell Center for Regenerative Biology and Tissue Engineering and National Xenopus Resource Marine Biological Laboratory  Woods Hole Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre L. P.","family":"Tavares","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology George Washington University School of Medicine and Health Sciences  Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikko\u2010Ideen","family":"Shaidani","sequence":"additional","affiliation":[{"name":"Eugene Bell Center for Regenerative Biology and Tissue Engineering and National Xenopus Resource Marine Biological Laboratory  Woods Hole Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Wlizla","sequence":"additional","affiliation":[{"name":"Eugene Bell Center for Regenerative Biology and Tissue Engineering and National Xenopus Resource Marine Biological Laboratory  Woods Hole Massachusetts USA"},{"name":"Embryology Department Charles River Laboratories  Wilmington Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4192-1087","authenticated-orcid":false,"given":"Sally A.","family":"Moody","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology George Washington University School of Medicine and Health Sciences  Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Horb","sequence":"additional","affiliation":[{"name":"Eugene Bell Center for Regenerative Biology and Tissue Engineering and National Xenopus Resource Marine Biological Laboratory  Woods Hole Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04716.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2015.11.003"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1025-06.2006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01516"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2020-107459"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.09.025"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.02.031"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098186"},{"key":"e_1_2_6_11_1","first-page":"64","article-title":"Ovarian hyperstimulation syndrome in gonadotropin\u2010treated laboratory South African clawed frogs (Xenopus laevis)","volume":"46","author":"Green S. L.","year":"2007","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01773"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(97)00158-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.08.020"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089695"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.80"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-1878(200007)22:7<616::AID-BIES4>3.0.CO;2-R"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2007.01.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.07.103"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8335-4"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(03)00065-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa012"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8784-9_1"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3543"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22484"},{"key":"e_1_2_6_26_1","unstructured":"Nieuwkoop P. D. Faber F. (1994).Normal Table of Xenopus laevis. New York: Garland Publishing."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00943"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17666"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.05.523"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106146"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20714"},{"key":"e_1_2_6_32_1","unstructured":"Smith R.J.(2018).Branchiotorenal Spectrum Disorders. 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