{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:47:32Z","timestamp":1753868852465,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Early growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies and pathophysiologies. Whole\u2010body EGR1 knockouts in mice (<jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>KO<\/jats:italic><\/jats:sup>) have advanced our understanding of EGR1 function in an in vivo context. To extend the utility of the mouse to investigate EGR1 responses in a tissue\u2010 and\/or cell\u2010type\u2010specific manner, we generated a mouse model in which exon 2 of the mouse <jats:italic>Egr1<\/jats:italic> gene is floxed by <jats:italic>CRISPR\/Cas9<\/jats:italic> engineering. The floxed <jats:italic>Egr1<\/jats:italic> alleles (<jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>f\/f<\/jats:italic><\/jats:sup>) are designed to enable spatiotemporal control of <jats:italic>Cre<\/jats:italic>\u2010mediated EGR1 ablation in the mouse. To confirm that the <jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>f\/f<\/jats:italic><\/jats:sup> alleles can be abrogated using a <jats:italic>Cre<\/jats:italic> driver, we crossed the <jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>f\/f<\/jats:italic><\/jats:sup> mouse with a global <jats:italic>Cre<\/jats:italic> driver to generate the <jats:italic>Egr1<\/jats:italic> conditional knockout (<jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>d\/d<\/jats:italic><\/jats:sup>) mouse in which EGR1 expression is ablated in all tissues. Genetic and protein analysis confirmed the absence of exon 2 and loss of EGR1 expression in the <jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>d\/d<\/jats:italic><\/jats:sup> mouse, respectively. Moreover, the <jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>d\/d<\/jats:italic><\/jats:sup> female exhibits overt reproductive phenotypes previously reported for the <jats:italic>Egr1<\/jats:italic><jats:sup><jats:italic>KO<\/jats:italic><\/jats:sup> mouse. Therefore, studies described in this short technical report underscore the potential utility of the murine <jats:italic>Egr1<\/jats:italic> floxed allele to further resolve EGR1 function at a tissue\u2010 and\/or cell\u2010type\u2010specific level.<\/jats:p>","DOI":"10.1002\/dvg.23515","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T04:12:54Z","timestamp":1679544774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A <scp>CRISPR<\/scp>\/Cas9\u2010engineered mouse carrying a conditional knockout allele for the early growth response\u20101 transcription factor"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4083-6331","authenticated-orcid":false,"given":"Vineet K.","family":"Maurya","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza Houston Texas 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2596-3183","authenticated-orcid":false,"given":"Yan","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza Houston Texas 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8750-6933","authenticated-orcid":false,"given":"Denise G.","family":"Lanza","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  One Baylor Plaza Houston Texas 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8475-8828","authenticated-orcid":false,"given":"Jason D.","family":"Heaney","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  One Baylor Plaza Houston Texas 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-636X","authenticated-orcid":false,"given":"John P.","family":"Lydon","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  One Baylor Plaza Houston Texas 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000059711"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2011.03.005"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:REMD.0000032413.88756.ee"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1026339111278"},{"key":"e_1_2_6_6_1","first-page":"409","volume-title":"Pathology of the mouse: Reference and atlas","author":"Davis B. 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