{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:47:36Z","timestamp":1753868856125,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["#81974229"],"award-info":[{"award-number":["#81974229"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Heterozygous mutation of <jats:italic>CHD7<\/jats:italic> gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of <jats:italic>Chd7<\/jats:italic> in vivo, we generated a <jats:italic>Chd7\u2010P2A\u2010iCreERT2\u2010P2A\u2010tdTomato<\/jats:italic> (in short, <jats:italic>Chd7\u2010CT\u2010tdT<\/jats:italic>) knockin mouse line using the CRISPR\/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The <jats:italic>Chd7\u2010CT\u2010tdT<\/jats:italic> reporter gene could accurately reflect both the dynamic expression pattern of endogenous <jats:italic>Chd7<\/jats:italic> during neurodevelopment and cell\u2010type specific expression in the brain and eye. The recombination efficiency of <jats:italic>Chd7\u2010CT\u2010tdT<\/jats:italic> in postnatal cerebellum is very high. Moreover, lineage tracing experiment showed that <jats:italic>Chd7<\/jats:italic> is expressed in intestinal stem cells. In summary, the newly constructed <jats:italic>Chd7\u2010CT\u2010tdT<\/jats:italic> mouse line provide a useful tool to study the function of <jats:italic>Chd7<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/dvg.23575","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:44:13Z","timestamp":1700667853000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation and characterization of <scp><i>Chd7\u2010iCreERT2\u2010tdTomato<\/i><\/scp> mice"],"prefix":"10.1002","volume":"62","author":[{"given":"Zi'ang","family":"Han","sequence":"first","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuxi","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-2144","authenticated-orcid":false,"given":"Weijun","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College Fudan University  Shanghai China"},{"name":"State\u2010level Regional Children's Medical Center, Children's Hospital of Fudan University at Xiamen (Xiamen Children's Hospital) Fujian Provincial Key Laboratory of Neonatal Diseases  Xiamen 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