{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T02:11:51Z","timestamp":1787191911152,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DE022503"],"award-info":[{"award-number":["R01 DE022503"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DE012711"],"award-info":[{"award-number":["R01 DE012711"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB\u20101818107"],"award-info":[{"award-number":["MCB\u20101818107"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Tfap2b, a pivotal transcription factor, plays critical roles within neural crest cells and their derived lineage. To unravel the intricate lineage dynamics and contribution of these Tfap2b+ cells during craniofacial development, we established a <jats:italic>Tfap2b\u2010CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup> knock\u2010in transgenic mouse line using the CRISPR\u2010Cas9\u2010mediated homologous direct repair. By breeding with tdTomato reporter mice and initiating Cre activity through tamoxifen induction at distinct developmental time points, we show the <jats:italic>Tfap2b<\/jats:italic> lineage within the key neural crest\u2010derived domains, such as the facial mesenchyme, midbrain, cerebellum, spinal cord, and limbs. Notably, the migratory neurons stemming from the dorsal root ganglia are visible subsequent to Cre activity initiated at E8.5. Intriguingly, Tfap2b+ cells, serving as the progenitors for limb development, show activity predominantly commencing at E10.5. Across the mouse craniofacial landscape, Tfap2b exhibits a widespread presence throughout the facial organs. Here we validate its role as a marker of progenitors in tooth development and have confirmed that this process initiates from E12.5. Our study not only validates the <jats:italic>Tfap2b\u2010CreER<\/jats:italic><jats:sup><jats:italic>T2<\/jats:italic><\/jats:sup> transgenic line, but also provides a powerful tool for lineage tracing and genetic targeting of <jats:italic>Tfap2b<\/jats:italic>\u2010expressing cells and their progenitor in a temporally and spatially regulated manner during the intricate process of development and organogenesis.<\/jats:p>","DOI":"10.1002\/dvg.23582","type":"journal-article","created":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T06:33:03Z","timestamp":1702103583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Generation of tamoxifen\u2010inducible <i>Tfap2b\u2010CreER<sup>T2<\/sup><\/i> mice using <scp>CRISPR\u2010Cas9<\/scp>"],"prefix":"10.1002","volume":"62","author":[{"given":"Mingyi","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Craniofacial Molecular Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifan","family":"Feng","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Z.","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2477-7247","authenticated-orcid":false,"given":"Yang","family":"Chai","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.03.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110234"},{"key":"e_1_2_7_4_1","first-page":"303","article-title":"Peculiar facies with short philtrum, duck\u2010bill lips, ptosis and low\u2010set ears\u2014A new syndrome?","volume":"14","author":"Char F.","year":"1978","journal-title":"Birth Defects Original Article 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