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We previously discovered unexpected expression of the commonly used <jats:italic>dilp2\u2010GAL4<\/jats:italic> line in tracheal tissue which significantly impacted growth phenotypes. We realized that few <jats:italic>GAL4<\/jats:italic> lines have been thoroughly characterized, particularly when considering transient activity that may have significant impact on phenotypic readouts. Here, we characterized a further subset of 12 reportedly tissue\u2010specific <jats:italic>GAL4<\/jats:italic> lines commonly used in genetic studies of development, growth, endocrine regulation, and metabolism. Ten out of 12 <jats:italic>GAL4<\/jats:italic> lines exhibited ectopic activity in other larval tissues, with seven being active in the larval trachea. Since this ectopic activity may result in phenotypes that do not depend on the manipulation in the intended target tissue, it is recommended to carefully analyze the outcome while taking this aspect into consideration.<\/jats:p>","DOI":"10.1002\/dvg.23600","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T07:09:41Z","timestamp":1714115381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Ectopic expression in commonly used transgenic <i>Drosophila<\/i><scp>GAL4<\/scp> driver lines"],"prefix":"10.1002","volume":"62","author":[{"given":"Mattias","family":"Winant","sequence":"first","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics KU Leuven \u2013 University of Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Buhler","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics KU Leuven \u2013 University of Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4530-4561","authenticated-orcid":false,"given":"Patrick","family":"Callaerts","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics KU Leuven \u2013 University of Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116467"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/163.1.203"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/533452a"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.2.401"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.165654"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.170039"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006346"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00205"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(03)00713-x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.01.092"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1800"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-583-1_3"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1356"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/332853a0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyac035"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.152710"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.09.010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.103109rk"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4238\/2012.August.6.4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.6311"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270050241"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2020.603397"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.08.028"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020180"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.09.017"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2012.00049"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/3.5.422"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.081968"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001160"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.036723"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac6767"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.120.401676"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T07:53:15Z","timestamp":1714204395000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/dvg.23600"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23600","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2024-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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