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Its overexpression has been shown to induce neurite outgrowth in embryonic neurons and to promote retinal ganglion cell (RGC) survival and axonal regrowth in a mouse optic nerve crush model. In order to evaluate the functions of endogenous Armcx1 <jats:italic>in vivo<\/jats:italic>, we have created a conditional <jats:italic>Armcx1<\/jats:italic> knockout mouse line in which the entire coding region of the <jats:italic>Armcx1<\/jats:italic> gene is flanked by <jats:italic>loxP<\/jats:italic> sites. This <jats:italic>Armcx1<\/jats:italic><jats:sup><jats:italic>fl<\/jats:italic><\/jats:sup> line was crossed with mouse strains in which Cre recombinase expression is driven by the promoters for <jats:italic>\u03b2\u2010actin<\/jats:italic> and <jats:italic>Six3<\/jats:italic>, in order to achieve deletion of <jats:italic>Armcx1<\/jats:italic> globally and in retinal neurons, respectively. Having confirmed deletion of the gene, we proceeded to characterize the abundance and morphology of RGCs in <jats:italic>Armcx1<\/jats:italic> knockout mice aged to 15\u2009months. Under normal physiological conditions, no evidence of aberrant retinal or optic nerve development or RGC degeneration was observed in these mice. The <jats:italic>Armcx1<\/jats:italic><jats:sup><jats:italic>fl<\/jats:italic><\/jats:sup> mouse should be valuable for future studies investigating mitochondrial morphology and transport in the absence of Armcx1 and in determining the susceptibility of Armcx1\u2010deficient neurons to degeneration in the setting of additional heritable or environmental stressors.<\/jats:p>","DOI":"10.1002\/dvg.23615","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T07:39:00Z","timestamp":1723621140000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Generation of an Armcx1 Conditional Knockout Mouse"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8683-0475","authenticated-orcid":false,"given":"Cora\u00a0L.","family":"Bright","sequence":"first","affiliation":[{"name":"University Program in Genetics and Genomics Duke University Graduate School  Durham North Carolina USA"},{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard\u00a0M.","family":"Bomze","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mantu","family":"Bhaumik","sequence":"additional","affiliation":[{"name":"Department of Neurology, F.M. Kirby Neurobiology Center Harvard Medical School, Boston Children's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy\u00a0N.","family":"Kay","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"},{"name":"Department of Neurobiology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Cartoni","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"},{"name":"Department of Pharmacology and Cancer Biology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-7983","authenticated-orcid":false,"suffix":"III","given":"Sidney\u00a0M.","family":"Gospe","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Duke University School of Medicine  Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162229"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2003.10.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.10.060"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3839"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<130::AID-GENE9>3.0.CO;2-I"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200601067"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2017.08.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1997.062.01.021"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/2024.01.24.577060"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2024.03.009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2023.106228"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.10.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1342"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34241"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2010.07.010"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a011304"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.11.027"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201073353\u20100"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.67"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2023.137300"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.23615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T12:42:43Z","timestamp":1724935363000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.23615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/dvg.23615"],"URL":"https:\/\/doi.org\/10.1002\/dvg.23615","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"2024-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23615"}}