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Tens of millions of patients worldwide suffer from heart injuries and other types of heart disease. Neonatal mammalian hearts and certain adult vertebrate species, such as zebrafish, can fully regenerate after myocardial injury. However, the adult mammalian heart is unable <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/hyperlink\">to<\/jats:ext-link> regenerate the damaged myocardium. scRNA\u2010seq provides many new insights into pathological and normal hearts and facilitates our understanding of cellular responses to cardiac injury and repair at different stages, which may provide critical clues for effective therapies for adult heart regeneration. In this review, we summarize the application of scRNA\u2010seq in heart development and regeneration and describe how important molecular mechanisms can be harnessed to promote heart regeneration.<\/jats:p>","DOI":"10.1002\/dvg.70013","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T18:28:06Z","timestamp":1745951286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Application of <scp>scRNA<\/scp>\u2010Seq in Heart Development and Regeneration"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7671-6267","authenticated-orcid":false,"given":"Junying","family":"Gao","sequence":"first","affiliation":[{"name":"Institute of Evolution &amp; Marine Biodiversity, Ocean University of China  Qingdao 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