{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T08:24:40Z","timestamp":1763540680796,"version":"3.45.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Astrocytes are a major glial cell type, playing multiple roles in the development, function, and pathogenesis of the brain. Accordingly, neuronal\u2013astrocyte communication is an important research area. However, because these cell types share the same developmental origin, selective manipulation of each cell type is needed for precise mechanistic understanding. Here, we generated two new Cre driver lines for selective gene manipulation in astrocytes: Slc7a10\u2010IRES\u2010Cre and Aldh1l1\u2010IRES\u2010Cre. An internal ribosome entry site (IRES)\u2010Cre cassette was knocked\u2010in to the 3\u2032\u2010untranslated region of the solute carrier family 7 member 10 (\n                    <jats:italic>Slc7a10<\/jats:italic>\n                    ) or aldehyde dehydrogenase 1 family member L1 (\n                    <jats:italic>Aldh1l1<\/jats:italic>\n                    ) locus without disrupting gene function. The Slc7a10\u2010IRES\u2010Cre line underwent highly selective recombination in astrocytes of the brain, apart from choroid plexus epithelial cells. The onset of recombination began after completion of differentiation in the astrocyte lineage. By contrast, the Aldh1l1\u2010IRES\u2010Cre line began recombination during astrocyte differentiation at early postnatal stages. Some leaky expression was observed in the oligodendrocyte lineage, probably due to early onset of Cre expression in an uncommitted glial progenitor state. Together, the combination of the two deleter lines with distinct temporal Cre expression patterns serves as valuable tools to understand the development and function of astrocytes.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70025","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T08:41:28Z","timestamp":1756802488000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation of Astrocyte\u2010Selective Cre Driver Lines With Distinct Onsets of Recombination Activity During Development"],"prefix":"10.1002","volume":"63","author":[{"given":"Yukina","family":"Izumi","sequence":"first","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoya","family":"Nakatani","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harumi","family":"Takai","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"},{"name":"Research and Development Department, Cyfuse Biomedical K.K.  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Kumai","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Kamisako","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Ishizaki","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7775-7433","authenticated-orcid":false,"given":"Yuichi","family":"Ono","sequence":"additional","affiliation":[{"name":"Eisai Co. Ltd. Kobe Research Laboratories  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.22405.1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.056"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.22.14.5100\u20105113.2002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4178\u201007.2008"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.12.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35592"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201010428\u20101"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1340"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10959"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1709\u201008.2008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264\u2010019\u201000451\u2010z"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4043"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3404\u201007.2007"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0896\u20106273(03)00116\u20108"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22836"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.188326.112"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20350"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04139"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010015\u20101513\u20101"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20570"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.11.030"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1280"},{"issue":"4","key":"e_1_2_6_24_1","first-page":"347","article-title":"Embryonic Stem Cells Derived From C57BL\/6J and C57BL\/6N Mice","volume":"58","author":"Tanimoto Y.","year":"2008","journal-title":"Comparative Medicine"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.077214"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583\u2010020\u20100264\u20108"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1860\u201014.2014"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10008"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T08:19:56Z","timestamp":1763540396000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.70025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/dvg.70025"],"URL":"https:\/\/doi.org\/10.1002\/dvg.70025","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"type":"print","value":"1526-954X"},{"type":"electronic","value":"1526-968X"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70025"}}