{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T02:45:56Z","timestamp":1766976356319,"version":"3.48.0"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2704700"],"award-info":[{"award-number":["2022YFC2704700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702400"],"award-info":[{"award-number":["2022YFC2702400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702500"],"award-info":[{"award-number":["2022YFC2702500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171660"],"award-info":[{"award-number":["82171660"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470906"],"award-info":[{"award-number":["32470906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82288102"],"award-info":[{"award-number":["82288102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The placenta plays an essential role during pregnancy in mammals, with the placental endocrine trophoblast subtypes secreting many growth factors and cytokines to promote fetal growth and maternal adaptation. These endocrine cells, including trophoblast giant cells (TGCs), glycogen trophoblast cells (GlyTs) and spongiotrophoblast cells (SpTs), are mainly derived from\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    \u2010positive (\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    ) trophoblast cells primarily located in the ectoplacental cone (EPC) and later junctional zone (JZ) of the mouse placenta. However, the mechanism driving\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    trophoblast cell differentiation and the functions of the factors secreted by these endocrine cells remain largely unknown. In the present study, we generated the\n                    <jats:italic>Tpbp\u03b1\u2010iCre\u2010EGFP<\/jats:italic>\n                    knock\u2010in mice with codon\u2010improved Cre recombinase (iCre) inserted into the endogenous locus of the\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    gene. To examine the specificity and efficiency of iCre recombinase, we crossed the\n                    <jats:italic>Tpbp\u03b1\u2010iCre\u2010EGFP<\/jats:italic>\n                    mice with\n                    <jats:italic>ROSA26Sor<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>tm9(CAG\u2010tdTomato)Hze<\/jats:italic>\n                    <\/jats:sup>\n                    reporter mice. The co\u2010expression of EGFP and Td\u2010tomato was detected obviously in the EPC at E8.5 and E9.5, and in the JZ at E13.5. Meanwhile, employing lineage tracing and in\u00a0situ hybridization, we demonstrated that\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    trophoblast cells could differentiate into SpTs, GlyTs, maternal blood canal (C)\u2010TGCs, parietal (P)\u2010TGCs, and spiral artery\u2010associated (Spa)\u2010TGCs. In addition, no\n                    <jats:italic>Tpbp\u03b1<\/jats:italic>\n                    expression or iCre recombinase activity was detected in other organs examined, indicating the specificity of the iCre activity in placental trophoblast cells. In summary, we successfully generated the\n                    <jats:italic>Tpbp\u03b1\u2010iCre\u2010EGFP<\/jats:italic>\n                    knock\u2010in mice with enhanced Cre recombinase for modulating specific genes and investigating their functions during pregnancy.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70034","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T08:49:16Z","timestamp":1765183756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation of\n                    <i>Tpbp\u03b1\u2010iCre\u2010EGFP<\/i>\n                    Knock\u2010In Mice for Trophoblast Subtype\u2010Specific Gene Manipulation"],"prefix":"10.1002","volume":"63","author":[{"given":"Shaoqiang","family":"Ban","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang'e","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengqi","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangbo","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-324X","authenticated-orcid":false,"given":"Haibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"},{"name":"State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhua","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Fujian Provincial Key Laboratory of Reproductive Health Research, The First Affiliated Hospital of Xiamen University, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2024"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201049930\u20106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010019\u20100169\u20104"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.07.036"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.066589"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23101"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.12a.1639"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010019\u20101683\u20101"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2014.12.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35080570"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.01.009"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.163428"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00570"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010022\u201003655\u20104"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029236"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216206120"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T02:42:51Z","timestamp":1766976171000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.70034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/dvg.70034"],"URL":"https:\/\/doi.org\/10.1002\/dvg.70034","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"type":"print","value":"1526-954X"},{"type":"electronic","value":"1526-968X"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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