{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:46:51Z","timestamp":1785826011791,"version":"3.56.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100011671","name":"G. Harold and Leila Y. Mathers Foundation","doi-asserted-by":"publisher","award":["MF\u20102203\u201002413"],"award-info":[{"award-number":["MF\u20102203\u201002413"]}],"id":[{"id":"10.13039\/100011671","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK080745"],"award-info":[{"award-number":["R01DK080745"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bicaudal C1 (Bicc1) encodes an RNA\u2010binding protein critical for many organ development and epithelial tissue homeostasis. Bicc1 null mutations have been shown to lead to the development of polycystic kidney disease (PKD) and death at an early prenatal stage. To elucidate the tissue\u2010specific functions of Bicc1, we engineered two independent conditional knockout (cKO) mouse lines targeting distinct exonic regions of the gene. The first line was generated using a traditional embryonic stem (ES) cell\u2010based approach, wherein loxP sites were inserted flanking exon 4 (E4), enabling Cre\u2010mediated excision of a functionally essential coding region. The second line was created using CRISPR\/Cas9 genome editing, introducing loxP sites around both exon 4 and exon 5 (E4\u20105) in a double\u2010step zygote injection strategy. Both alleles were validated by PCR genotyping, sequencing, and functional recombination was confirmed via a tissue\u2010specific Cre driver. These independent cKO models provide a robust platform for dissecting the role of\n                    <jats:italic>Bicc1<\/jats:italic>\n                    in specific tissues and developmental stages, and offer new avenues for studying the mechanistic basis of PKD and other Bicc1\u2010related pathologies.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70038","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T08:51:46Z","timestamp":1767948706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Generation of Mice Harboring\n                    <i>Bicc1<\/i>\n                    Conditional Null Alleles"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9383-341X","authenticated-orcid":false,"given":"Chia\u2010Feng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Heart, Blood and Kidney Research Cleveland Clinic Research, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1207-7027","authenticated-orcid":false,"given":"Steven","family":"Leon","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood and Kidney Research Cleveland Clinic Research, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9607-5433","authenticated-orcid":false,"given":"Isabella","family":"Herrig","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood and Kidney Research Cleveland Clinic Research, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-7975","authenticated-orcid":false,"given":"Oliver","family":"Wessely","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood and Kidney Research Cleveland Clinic Research, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8335-735X","authenticated-orcid":false,"given":"W. H. Wilson","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood and Kidney Research Cleveland Clinic Research, Cleveland Clinic  Cleveland Ohio USA"},{"name":"Department of Cardiovascular Medicine, Heart and Vascular Institute Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"issue":"2","key":"e_1_2_7_2_1","first-page":"96","article-title":"Knockdown of Bicaudal C in Zebrafish (\n                     Danio rerio\n                  ) Causes Cystic Kidneys: A Nonmammalian Model of Polycystic Kidney Disease","volume":"60","author":"Bouvrette D. J.","year":"2010","journal-title":"Comparative Medicine"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-002-2241-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.981696"},{"key":"e_1_2_7_5_1","volume-title":"Body Weight Chart","author":"JAX","year":"2005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1995.tb07196.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.038174"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.131359"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn045"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.013537"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2019.109499"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.00341\u201015"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.8.4855"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.asn.0000016444.90348.50"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.04.030"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.046045"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.10.2053"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0925\u20104773(00)00568\u20102"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70038","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvg.70038","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:09:23Z","timestamp":1773029363000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.70038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/dvg.70038"],"URL":"https:\/\/doi.org\/10.1002\/dvg.70038","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]},"assertion":[{"value":"2025-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70038"}}