{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T12:52:08Z","timestamp":1782478328902,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R21CA267006"],"award-info":[{"award-number":["R21CA267006"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R50CA283804"],"award-info":[{"award-number":["R50CA283804"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21CA"],"award-info":[{"award-number":["R21CA"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>TWIST1<\/jats:italic>\n                    encodes a highly conserved basic helix\u2013loop\u2013helix transcription factor essential for embryonic development from Drosophila to humans. TWIST1 activity is regulated by post\u2010translational modifications, including phosphorylation during development and cancer metastasis. Recent cancer studies identified acetylation of lysines K73 and K76 as a novel regulatory modification that shifts TWIST1 from a repressive to an activating state during epithelial\u2010to\u2010mesenchymal transition (EMT). However, the developmental and in\u00a0vivo functions of TWIST1 acetylation remain unknown. To investigate the physiological role, we generated the first acetyl\u2010deficient\n                    <jats:italic>Twist1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>K73,76R K73,76R<\/jats:italic>\n                    <\/jats:sup>\n                    and acetyl\u2010mimic\n                    <jats:italic>Twist1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>K73,76Q K73,76Q<\/jats:italic>\n                    <\/jats:sup>\n                    mouse models using CRISPR\/Cas9\u2010mediated genome editing. Targeted sequencing confirmed substitutions, and founders were backcrossed onto a C57BL\/6J genetic background. Phenotypic analysis revealed that\n                    <jats:italic>Twist1<\/jats:italic>\n                    acetyl\u2010deficient mice exhibited highly penetrant craniofacial abnormalities, including severe mandibular hypoplasia, mandibular bone fusion, replacement of premaxillary, maxillary, and palatine bones with cartilage, ectopic cartilage\u2010like structures, ocular malformations, and reduced skull mineralization. In contrast, acetyl\u2010mimic mutant embryos displayed milder craniofacial defects characterized by reduced mandibular condylar processes and palatine bones, along with decreased skull mineralization. These findings demonstrate that TWIST1 acetylation at K73 and K76 plays a crucial role in normal craniofacial skeletogenesis, supporting prior cancer studies showing reduced oncogenic activity following loss of TWIST1 acetylation.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70062","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:30:00Z","timestamp":1781757000000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation and Characterization of\n                    <scp>\n                      <i>Twist1<\/i>\n                    <\/scp>\n                    Acetyl\u2010Mimic and Acetyl\u2010Deficient Mouse Models"],"prefix":"10.1002","volume":"64","author":[{"given":"Mary","family":"Elmeniawi","sequence":"first","affiliation":[{"name":"Department of Diagnostic and Biomedical Sciences, Center for Craniofacial Research, School of Dentistry University of Texas Health Science Center at Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Biomedical Sciences, Center for Craniofacial Research, School of Dentistry University of Texas Health Science Center at Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunjan A.","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Biomedical Sciences, Center for Craniofacial Research, School of Dentistry University of Texas Health Science Center at Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2199-3828","authenticated-orcid":false,"given":"Walid D.","family":"Fakhouri","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Biomedical Sciences, Center for Craniofacial Research, School of Dentistry University of Texas Health Science Center at Houston  Houston Texas USA"},{"name":"Department of Pediatrics, McGovern Medical School University of Texas Health Science Center at Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.12.002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.200068"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.08.016"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.034"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20332"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.6.686"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0197\u201042"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201006310\u2010z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62873"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.00663\u201019"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986708785908987"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1525"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702613200"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.081679"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.can\u201010\u20102914"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5201507"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019251"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0699"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.13"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.04.037"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.06.021"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202356902"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvg.70062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T12:44:25Z","timestamp":1782477865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.70062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/dvg.70062"],"URL":"https:\/\/doi.org\/10.1002\/dvg.70062","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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