{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:21:16Z","timestamp":1787826076556,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102026\u201025471326"],"award-info":[{"award-number":["RS\u20102026\u201025471326"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2019H1A2A1075288"],"award-info":[{"award-number":["2019H1A2A1075288"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Transgenic reporters offer valuable opportunities to investigate the genetic and cellular basis of embryonic development in live organisms. While the specificity of transgenic reporters is essential for their effectiveness in developmental studies, many existing transgenic muscle reporters used for craniofacial muscle development in zebrafish also label trunk skeletal and cardiac muscles, as well as other head tissues. Here, we report the identification of a new transgenic reporter marking craniofacial muscles,\n                    <jats:italic>Tg(nkx2.3:Alcama\u2010GFP)<\/jats:italic>\n                    , in zebrafish. This transgenic reporter exhibits Alcama\u2010GFP expression in growing muscle fibers at embryonic stages and in grown muscle fibers at larval stages. However, endogenous\n                    <jats:italic>nkx2.3<\/jats:italic>\n                    transcripts are barely detected in growing craniofacial muscle fibers by in\u00a0situ hybridization, and\n                    <jats:italic>nkx2.3<\/jats:italic>\n                    mutants display fairly normal ventral jaw muscles adjacent to the lower jaw cartilages and extraocular muscles adjacent to the eye. Although an essential role for Nkx2.3 in craniofacial muscle formation remains to be determined, the\n                    <jats:italic>Tg(nkx2.3:Alcama\u2010GFP)<\/jats:italic>\n                    craniofacial muscle reporter provides better opportunities for the precise analysis of craniofacial muscle development at the cellular and genomic levels.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70069","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:30:13Z","timestamp":1785821413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a Novel\n                    <i>nkx2.3<\/i>\n                    Transgenic Reporter Specifically Visualizing Craniofacial Muscle Development in Zebrafish"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1605-4990","authenticated-orcid":false,"given":"Yucheol","family":"Choe","sequence":"first","affiliation":[{"name":"Division of Applied Life Science Gyeongsang National University  Jinju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9451-1714","authenticated-orcid":false,"given":"Haewon","family":"Jeon","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science Gyeongsang National University  Jinju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1177-299X","authenticated-orcid":false,"given":"Sil","family":"Jin","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology and Biotechnology Research Center Gyeongsang National University  Jinju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0254-9438","authenticated-orcid":false,"given":"Chong Pyo","family":"Choe","sequence":"additional","affiliation":[{"name":"Plant Molecular Biology and Biotechnology Research Center Gyeongsang National University  Jinju Korea"},{"name":"Division of Life Science Gyeongsang National University  Jinju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22385"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.12.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.111740"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21928"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.015719"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201004402\u20106"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/332853a0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2501"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395\u2010014\u20100026\u20102"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2025.1482906"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2025.1652723"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10273"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.719"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007112"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.07.022"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201036612\u2010y"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9842"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.100347"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.67"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.040972"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.3.483"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.15.2945"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201038647\u20107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.049700"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24098314"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.78170"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13227\u2010019\u20100128\u20103"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.10124"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21915"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvg.70069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.70069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T09:49:23Z","timestamp":1787824163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.70069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/dvg.70069"],"URL":"https:\/\/doi.org\/10.1002\/dvg.70069","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70069"}}