{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:02:27Z","timestamp":1790744547893,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Reverse breeding (RB), a novel plant breeding approach, helps to identify the parental homozygous lines for any uncharacterized but useful heterozygous plant that a plant breeder needs utmost. For this, genetic engineering at meiotic cell division, particularly meiosis\u2010I, must achieve the right combination and complement homozygous parental lines for recreating the desired hybrid. In this technology, meiotic cross\u2010over is suppressed to stop the recombination at the chromosome level in the selected heterozygous plants. The success of RB in crops depends on the efficiency and effectiveness of doubled haploid (DH) technology, which is needed to produce diploid (2\n                    <jats:italic>n<\/jats:italic>\n                    ) plants from haploid gametes (\n                    <jats:italic>n<\/jats:italic>\n                    ) after diploidization. The review initially highlights the current status and requirement of RB in addressing global challenges related to food and nutritional security. It then discusses the different mechanisms to engineer meiosis to suppress cross\u2010over and the fixation of nonrecombinant chromosomes through DH technology. The review further examines immediate applications of RB and the factors influencing its efficiency. The advantages and limitations of RB are also discussed, along with its potential applications in future crop improvement. In conclusion, RB represents a promising plant breeding approach for overcoming prevailing challenges through the development of crop varieties with improved disease resistance, climate resilience, nutritional quality, and other traits relevant to sustainable agriculture and human needs.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70070","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:53:36Z","timestamp":1790744016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reverse Breeding: A Breakthrough Technique for Genetic Fixation and Hybrid Reconstruction"],"prefix":"10.1002","volume":"64","author":[{"given":"Manoj Kumar","family":"Patel","sequence":"first","affiliation":[{"name":"ICAR\u2010Indian Agricultural Research Institute  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-355X","authenticated-orcid":false,"given":"Vijay Kamal","family":"Meena","sequence":"additional","affiliation":[{"name":"Agriculture Research Sub\u2010Station (Sumperpur) Agriculture University  Jodhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3328-3329","authenticated-orcid":false,"given":"Sanjay","family":"Kumar","sequence":"additional","affiliation":[{"name":"CSK Himachal Pradesh Krishi Vishwavidyalaya  Palampur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7323-7020","authenticated-orcid":false,"given":"Pawan","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute of Arid Horticulture  Bikaner India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6898-9861","authenticated-orcid":false,"given":"Subhash","family":"Chand","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Grassland and Fodder Research Institute  Jhansi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankit","family":"Malik","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Agricultural Research Institute  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1413-4994","authenticated-orcid":false,"given":"Kadam","family":"Kiran","sequence":"additional","affiliation":[{"name":"Rani Lakshmi Bai Central Agricultural University Jhansi  Jhansi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharath Kumar","family":"Alam","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Research Institute for Dryland Agriculture  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjun Lal","family":"Ola","sequence":"additional","affiliation":[{"name":"Rani Lakshmi Bai Central Agricultural University Jhansi  Jhansi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0122-6234","authenticated-orcid":false,"given":"Girija","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Rani Lakshmi Bai Central Agricultural University Jhansi  Jhansi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2201-6113","authenticated-orcid":false,"given":"Rakesh","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Rani Lakshmi Bai Central Agricultural University Jhansi  Jhansi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"12","key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.1093\/emboj\/cdf285","article-title":"Homolog Interaction During Meiotic Prophase I in Arabidopsis Requires the SOLO DANCERS Gene Encoding a Novel Cyclin\u2010Like Protein","volume":"21","author":"Azumi Y.","year":"2002","journal-title":"EMBO Journal"},{"issue":"5","key":"e_1_2_13_3_1","doi-asserted-by":"crossref","first-page":"e105","DOI":"10.1371\/journal.pbio.0050105","article-title":"A Dominant, Recombination\u2010Defective Allele of Dmc1 Causing Male\u2010Specific Sterility","volume":"5","author":"Bannister L. 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