{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:39:02Z","timestamp":1792661942119,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271197"],"award-info":[{"award-number":["82271197"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81974175"],"award-info":[{"award-number":["81974175"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gonadotropin\u2010releasing hormone (GnRH) neurons play a key role in the hypothalamic\u2010pituitary\u2010gonadal axis. We generated a new\n                    <jats:italic>GnRH\u2010P2A\u2010iCreERT2\u2010P2A\u2010tdTomato<\/jats:italic>\n                    (hereafter referred to as the\n                    <jats:italic>GnRH\u2010CT\u2010tdT<\/jats:italic>\n                    line) knock\u2010in mouse line utilizing CRISPR\/Cas9 technology. Characterization analysis of this line revealed that tdTomato expression precisely recapitulates endogenous GnRH expression and iCreERT2 recombines in GnRH neurons with excellent specificity and sensitivity. Therefore, this newly constructed\n                    <jats:italic>GnRH\u2010CT\u2010tdT<\/jats:italic>\n                    mouse line provides a useful tool to perform genetic manipulation in GnRH neurons. As a proof\u2010of\u2010principle, we used this line to investigate the role of\n                    <jats:italic>Chd7<\/jats:italic>\n                    (Chromodomain helicase DNA\u2010binding protein 7), a gene with its loss\u2010of\u2010function associated with isolated gonadotropin\u2010releasing hormone deficiency (IGD). Surprisingly, ablation of\n                    <jats:italic>Chd7<\/jats:italic>\n                    in GnRH neurons does not affect the number of GnRH neurons, pubertal onset, and reproductive organ morphology. Therefore,\n                    <jats:italic>Chd7<\/jats:italic>\n                    is dispensable in GnRH neurons after they have initiated GnRH expression. In summary, the\n                    <jats:italic>GnRH\u2010CT\u2010tdT<\/jats:italic>\n                    mouse line is a valuable tool to study gene function in GnRH neurons and to dissect the pathogenesis of IGD.\n                  <\/jats:p>","DOI":"10.1002\/dvg.70072","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T08:42:43Z","timestamp":1792658563000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation and Validation of a\n                    <i>GnRH\u2010iCreERT2\u2010tdTomato<\/i>\n                    Mouse Line for Genetic Manipulation in\n                    <scp>GnRH<\/scp>\n                    Neurons"],"prefix":"10.1002","volume":"64","author":[{"given":"Shuhua","family":"Dong","sequence":"first","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi'ang","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"He","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Department Children's Hospital of Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0614-7068","authenticated-orcid":false,"given":"Dashi","family":"Qi","sequence":"additional","affiliation":[{"name":"Center for Clinical Research and Translational Medicine, Yangpu Hospital, School of Medicine, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-2144","authenticated-orcid":false,"given":"Weijun","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co\u2010laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University  Shanghai China"},{"name":"Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University  Shanghai China"},{"name":"Shanghai Key Laboratory of Gene Editing and Cell Therapy for Rare Diseases, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08733"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417438111"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.c.31585"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2009.158"},{"key":"e_1_2_9_6_1","first-page":"307","volume-title":"Handbook of Clinical Neurology","author":"Cariboni 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