{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:55:20Z","timestamp":1791204920625,"version":"4.1.0"},"reference-count":101,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["esajournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecological Applications"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Wildfires are a natural disturbance in dry ponderosa pine forest ecosystems, and are increasing in frequency and severity across western North America. The effects of wildfire on biotic and abiotic conditions have important implications for higher trophic levels, including native bees. We sought to quantify the responses of flowering plants to fire age and severity and determine the effects of these shifts on pollinator richness through time. We sequenced pollen from pan and blue vane traps deployed every 2\u2009weeks from April to August in 2022 and 2023 to quantify shifts in foraging resources for pollinator communities in landscapes aged 1\u20138\u2009years post\u2010fire, and in an unburned area. DNA metabarcoding pollen suspended in blue vane and pan traps revealed herbaceous perennials such as\n                    <jats:italic>Achillea millefolium<\/jats:italic>\n                    ,\n                    <jats:italic>Lupinus<\/jats:italic>\n                    spp., and\n                    <jats:italic>Phacelia hastata<\/jats:italic>\n                    to be key floral resources for post\u2010fire pollinator communities. Burn age and burn severity were the main drivers of plant\u2010pollinator richness, and plant species richness increased with burn age and burn severity. Bee species richness varied among burn severities, supporting management for patch mosaics and landscape heterogeneity as a fundamental principle of fire reintroduction. Our findings underscore the importance of bioinformatics threshold selection in plant list generation and highlight the role of burn age, burn severity, and plant richness in effectively conserving pollinator species across fire\u2010effected landscapes. This study also used the novel approach of collecting pollen suspended in trap solutions to quantify floral resources for insect pollinators, highlighting the utility of trap byproducts to provide insightful ecological information. Understanding post\u2010fire plant\u2010pollinator relationships is critical for successful conservation of dry forest angiosperm flora and bee faunas.\n                  <\/jats:p>","DOI":"10.1002\/eap.70325","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:22:05Z","timestamp":1791202925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>DNA<\/scp>\n                    metabarcoding reveals key floral resources for native pollinators in post\u2010fire landscapes"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9108-8697","authenticated-orcid":false,"given":"Autumn","family":"Maust","sequence":"first","affiliation":[{"name":"School of Environmental and Forest Sciences University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1794-6128","authenticated-orcid":false,"given":"Ellen M.","family":"Reese","sequence":"additional","affiliation":[{"name":"School of Environmental and Forest Sciences University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0754-407X","authenticated-orcid":false,"given":"Madeleine","family":"Strait","sequence":"additional","affiliation":[{"name":"Department of Biology University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0237-7963","authenticated-orcid":false,"given":"Patrick C.","family":"Tobin","sequence":"additional","affiliation":[{"name":"School of Environmental and Forest Sciences University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1523-1739.1998.97154.x","article-title":"The Potential Consequences of Pollinator Declines on the Conservation of Biodiversity and Stability of Food Crop Yields","volume":"12","author":"Allen\u2010Wardell G.","year":"1998","journal-title":"Conservation Biology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/boj.12385"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16112"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0282715"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2025.0643"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15675"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting Linear Mixed\u2010Effects Models Using lme4","volume":"67","author":"Bates D.","year":"2015","journal-title":"Journal of Statistical Software"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen-2015-0200"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14840"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.70026"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.32614\/RJ-2017-066"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido\/2009019"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016931"},{"issue":"4","key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/j.2326-1951.1996.tb03254.x","article-title":"The Pollination Crisis","volume":"36","author":"Buchmann S. L.","year":"1996","journal-title":"The Sciences"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00173139109427817"},{"key":"e_1_2_9_17_1","unstructured":"Burke Herbarium.2025.\u201cImage collection. Burke Herbarium.\u201dhttps:\/\/www.burkeherbarium.org\/imagecollection\/Accessed December 2025."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2006.08.029"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2011.07.019"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/86.5.577"},{"issue":"1","key":"e_1_2_9_22_1","article-title":"Causes and Extent of Declines among Native North American Invertebrate Pollinators: Detection, Evidence, and Consequences","volume":"5","author":"Cane J. H.","year":"2001","journal-title":"Conservation Ecology"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-0831.2003.tb00140.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2664.14063"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008613"},{"issue":"758","key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1086\/280955","article-title":"Regional Differentiation in Plant Species","volume":"75","author":"Clausen J.","year":"1941","journal-title":"The American Naturalist"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/03610929208830766"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2014.08.001"},{"key":"e_1_2_9_29_1","unstructured":"De C\u00e1ceres M. F.Jansen S.Endicott andN.Dell.2025.\u201cindicspecies: Relationship between species and groups of sites.\u201dhttps:\/\/cran.r-project.org\/web\/packages\/indicspecies\/index.html."},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.2894"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072016"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9615(1997)067[0345:SAAIST]2.0.CO;2"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16818"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2307\/3800909"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16682"},{"key":"e_1_2_9_36_1","volume-title":"Natural Vegetation of Oregon and Washington","author":"Franklin J. F.","year":"1973"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.2668"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2656.2002.00641.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1255957"},{"key":"e_1_2_9_40_1","unstructured":"Greenacre M. O.Nenadic andM.Friendly.2025.\u201cPackage \u2018ca\u2019: Simple Multiple and Joint Correspondence Analysis.\u201dhttps:\/\/cran.r-project.org\/web\/packages\/ca\/ca.pdf"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1890\/08-1792.1"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvs.12966"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2020.1191"},{"key":"e_1_2_9_44_1","unstructured":"Hartig F.2024.\u201cDHARMa: Residual Diagnostics for Hierarchical (Multi\u2010Level\/Mixed) Regression Models.\u201d R package version 0.4.7.\u201dhttps:\/\/cran.r-project.org\/web\/packages\/DHARMa\/vignettes\/DHARMa.html."},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134735"},{"key":"e_1_2_9_46_1","unstructured":"iNaturalistAvailable fromhttps:\/\/www.inaturalist.org. Accessed December 2025."},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1029\/2020RG000726"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/plb.12251"},{"key":"e_1_2_9_49_1","first-page":"LA\u20101\u201055 164","volume-title":"FIREMON: Fire Effects Monitoring and Inventory System. Gen. Tech. Rep. RMRS\u2010GTR\u2010164\u2010CD","author":"Key C. H.","year":"2006"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00265-008-0684-3"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.2307\/3798627"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/beheco\/12.4.447"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v082.i13"},{"key":"e_1_2_9_54_1","volume-title":"Attracting Native Pollinators: Protecting North America's Bees and Butterflies","author":"Mader E.","year":"2011"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112839109"},{"key":"e_1_2_9_56_1","volume-title":"American Wildlife and Plants: A Guide to Wildlife Food Habits: The Use of Trees, Shrubs, Weeds, and Herbs by Birds and Mammals of the United States","author":"Martin A. C.","year":"1961"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foreco.2026.123567"},{"key":"e_1_2_9_58_1","unstructured":"Maust A. E. M.Reese M.Strait andP. C.Tobin.2026b.\u201cData from: DNA Metabarcoding Reveals Key Floral Resources for Native Pollinators in Post\u2010Fire Landscapes.\u201d[Dataset]. Dryad.https:\/\/doi.org\/10.5061\/dryad.g4f4qrg60."},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.15560\/21.2.374"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v112.i01"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/jwmg.896"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1139\/z95-082"},{"key":"e_1_2_9_63_1","first-page":"3806","article-title":"The ITS2 Database","volume":"61","author":"Merget B.","year":"2012","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/2688-8319.12120"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rse.2006.12.006"},{"key":"e_1_2_9_66_1","volume-title":"Washington State University Collection Occurrence Dataset","author":"MT James Entomological Collection","year":"2026"},{"issue":"3","key":"e_1_2_9_67_1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1007\/BF02861079","article-title":"The Aerodynamics of Wind Pollination","volume":"51","author":"Niklas K. J.","year":"1985","journal-title":"The Botanical Review"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.3368\/er.28.3.280"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2699.1998.252166.x"},{"key":"e_1_2_9_70_1","unstructured":"Oksanen J. G. L.Simpson F.Guillaume Blanchet R.Kindt P.Legendre P. R.Minchin R. B.O'Hara et al.2024.\u201cVegan: Community Ecology Package.\u201dR Package Version 2.6\u20138."},{"key":"e_1_2_9_71_1","volume-title":"Melittoflora: Plant\u2010Pollinator Interactions Visualization Tool","author":"Oregon State University","year":"2025"},{"key":"e_1_2_9_72_1","first-page":"55","volume-title":"Measuring Arthropod Biodiversity: A Handbook of Sampling Methods","author":"Packer L.","year":"2020"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/rs11141735"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56333-8"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1139\/z84-354"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.13236"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2005.11.019"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agrformet.2019.107803"},{"key":"e_1_2_9_79_1","unstructured":"Qiagen.2016.\u201cDNeasy\u00ae Plant Mini Kit: Quick\u2010Start Protocol.\u201dRetrieved April 21 2025 fromhttps:\/\/www.qiagen.com"},{"key":"e_1_2_9_80_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2025"},{"key":"e_1_2_9_81_1","volume-title":"Selected Weeds of the United States","author":"Reed C. F.","year":"1970"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.1695"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/say021"},{"key":"e_1_2_9_84_1","doi-asserted-by":"crossref","DOI":"10.2737\/PNW-GTR-657","volume-title":"A Social History of Wild Huckleberry Harvesting in the Pacific Northwest","author":"Richards R.","year":"2006"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcbb.12531"},{"issue":"3","key":"e_1_2_9_86_1","doi-asserted-by":"crossref","first-page":"378","DOI":"10.2307\/1929320","article-title":"Revised List of Oligolectic Bees","volume":"7","author":"Robertson C.","year":"1926","journal-title":"Ecology"},{"issue":"5","key":"e_1_2_9_87_1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1017\/S0043174500033853","article-title":"Germination of Seeds of Common Yarrow (Achillea Millefolium) and its Herbicidal Control","volume":"25","author":"Robocker W. C.","year":"1977","journal-title":"Weed Science"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.151663"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1111\/icad.12243"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvs.12796"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1061-2971.2004.00262.x"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjps82-024"},{"key":"e_1_2_9_94_1","volume-title":"Chelan County Plant List","author":"Washington Native Plant Society","year":"2023"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42408-023-00200-8"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9658(1997)078[1105:COFBB]2.0.CO;2"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-023-05354-5"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-022-02164-9"},{"key":"e_1_2_9_99_1","first-page":"315","volume-title":"PCR Protocols: A Guide to Methods and Applications","author":"White T. J.","year":"1990"},{"key":"e_1_2_9_100_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham H.","year":"2016"},{"key":"e_1_2_9_101_1","volume-title":"treemapify: Draw Treemaps in 'ggplot2'","author":"Wilkins D.","year":"2023"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1111\/cobi.12150"}],"container-title":["Ecological Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/esajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eap.70325","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/esajournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/eap.70325","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/esajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eap.70325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:22:14Z","timestamp":1791202934000},"score":1,"resource":{"primary":{"URL":"https:\/\/esajournals.onlinelibrary.wiley.com\/doi\/10.1002\/eap.70325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":101,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/eap.70325"],"URL":"https:\/\/doi.org\/10.1002\/eap.70325","archive":["Portico"],"relation":{},"ISSN":["1051-0761","1939-5582"],"issn-type":[{"value":"1051-0761","type":"print"},{"value":"1939-5582","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70325"}}