{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:54:00Z","timestamp":1790736840464,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006147","name":"Office of Nuclear Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006147","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl J Eating Disorders"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Caseinolytic protease B (ClpB) produced by <jats:italic>Enterobacteria<\/jats:italic>, such as <jats:italic>Escherichia coli<\/jats:italic>, has been identified as a conformational mimetic of \u03b1\u2010melanocyte\u2010stimulating hormone (\u03b1\u2010MSH), an anorexigenic and anxiogenic neuropeptide. In mice, ClpB induces \u03b1\u2010MSH cross\u2010reactive antibodies and activates anorexigenic brain neurons. In patients with eating disorders (ED), anti\u2010ClpB and anti\u2010\u03b1\u2010MSH antibodies correlate with psychopathological traits. However, it is not known if ClpB is present in human plasma including ED patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma concentrations of ClpB were measured using a recently developed ClpB immunoassay in female patients with anorexia nervosa, bulimia nervosa, and binge\u2010eating disorder and compared with healthy participants, all characterized by the Eating Disorder Inventory\u20102 (EDI\u20102) scale.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that ClpB was readably detectable in plasma of healthy participants and ED patients and that its concentrations were elevated in ED patients, without significant differences in patient's subgroups. Plasma ClpB concentrations correlated with the EDI\u20102 scores, with \u03b1\u2010MSH as well as with plasma levels of anti\u2010ClpB and anti\u2010\u03b1\u2010MSH antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These data revealed that bacterial ClpB is naturally present in human plasma and that its concentrations can be elevated in ED patients and associated with ED\u2010related psychopathological traits. These results support a link between bacterial ClpB and the ED pathophysiology. \u00a9 2016 Wiley Periodicals, Inc. 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University, Normandy University Rouen 76000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsti","family":"Akkermann","sequence":"additional","affiliation":[{"name":"Department of Psychology Estonian Centre of Behavioural and Health Sciences Tartu 50409 Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anu","family":"J\u00e4rv","sequence":"additional","affiliation":[{"name":"Tartu University Clinics, Psychiatric Hospital, University of Tartu Tartu 50417 Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaanus","family":"Harro","sequence":"additional","affiliation":[{"name":"Department of Psychology Estonian Centre of Behavioural and Health Sciences Tartu 50409 Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"D\u00e9chelotte","sequence":"additional","affiliation":[{"name":"Nutrition, Gut and Brain Laboratory, Inserm UMR1073 Rouen 76183 France"},{"name":"Institute for 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