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However, the underlying cellular mechanisms remain inadequately elucidated, and interventions addressing these alterations are lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>This study employed a chronic activity\u2010based anorexia (ABA) rat model to investigate hippocampal glial and neuronal cell alterations, inflammation, and microbial modifications in the gut. Omega\u20103 fatty acids and a multi\u2010strain probiotic were examined as potentially protective agents. Cell count, proliferation, and apoptosis rates of microglia, neurons, astrocytes, and oligodendrocytes were analyzed in the hippocampus. Furthermore, local gene expression of pro\u2010inflammatory cytokines and microRNA was measured. The hippocampal volume was determined longitudinally using 7\u2010Tesla MRI scans before and after starvation. Finally, the fecal microbiome was analyzed to identify potential associations with brain and clinical characteristics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Results confirmed the previously described reduction in astrocytes and newly demonstrated a decrease in oligodendrocytes in the hippocampus. Increased levels of IBA1\u2010positive cells and pro\u2010inflammatory cytokines suggest microglial activation in this region. Administering omega\u20103 and probiotics to starved animals reduced neuroinflammation and microglial activation in the hippocampus, resulting in significantly increased neuronal cell counts in the omega\u20103 group. Microbiome composition was primarily affected by the ABA model, and to a lesser extent by omega\u20103 and probiotics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings support the involvement of microglial activation in the pathogenesis of AN, potentially relevant to hippocampal re\u2010learning and thus important for psychotherapy. Further, omega\u20103 and probiotics may serve as adjunct therapeutic strategies by modifying inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/eat.24574","type":"journal-article","created":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T16:13:28Z","timestamp":1760804008000},"page":"260-275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Influence of Omega\u20103 Fatty Acids and Probiotics on Hippocampal Inflammation and Glial Cells in a Chronic Anorexia Nervosa Rat Model"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6728-4888","authenticated-orcid":false,"given":"A. 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