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In regions like the Arctic, where ecosystems are particularly susceptible, marked changes are predicted in regional diversity, intensity, and patterns of infectious diseases. To understand such rapidly changing host\u2010pathogen dynamics and mitigate the impacts of novel pathogens, we need sensitive disease surveillance tools. We developed and validated a novel multiplexed, magnetic capture, and ddPCR tool for the surveillance of multiple pathogens in polar bears, a sentinel species that is considered susceptible to climate change and other stressors with a pan\u2010Arctic distribution. Through sequence\u2010specific magnetic capture, we concentrated five target template sequences from three zoonotic bacteria (\n                    <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>\n                    ,\n                    <jats:italic>Francisella tularensis<\/jats:italic>\n                    , and\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    complex) and two parasitic (\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    and\n                    <jats:italic>Trichinella<\/jats:italic>\n                    spp.) pathogens from large quantities (&lt;100\u2009g) of host tissue. We then designed and validated two multiplexed probe\u2010based ddPCR assays for the amplification and detection of the low\u2010concentration target DNA. Validations used 48 polar bear tissues (muscle and liver). We detected 14, 1, 3, 4, and 22 tissue positives for\n                    <jats:italic>E. rhusiopathiae<\/jats:italic>\n                    ,\n                    <jats:italic>F. tularensis<\/jats:italic>\n                    ,\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    complex,\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , and\n                    <jats:italic>Trichinella<\/jats:italic>\n                    spp., respectively. These multiplexed assays offer a rapid, specific tool for quantifying and monitoring the changing geographical and host distributions of pathogens relevant to human and animal health.\n                  <\/jats:p>","DOI":"10.1002\/ece3.10655","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T04:59:24Z","timestamp":1698728364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A new multiplexed magnetic capture\u2014Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance"],"prefix":"10.1002","volume":"13","author":[{"given":"Christina M.","family":"Tschritter","sequence":"first","affiliation":[{"name":"Department of Biology Queen's University  Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"V.\u00a0C.\u00a0de\u00a0Groot","sequence":"additional","affiliation":[{"name":"Department of Biology Queen's University  Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marsha","family":"Branigan","sequence":"additional","affiliation":[{"name":"Department of Environment and Natural Resources Government of the Northwest Territories  Inuvik Northwest Territories Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Dyck","sequence":"additional","affiliation":[{"name":"Department of Environment Government of Nunavut  Igloolik Nunavut Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengxin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biology Queen's University  Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1466-3668","authenticated-orcid":false,"given":"Stephen C.","family":"Lougheed","sequence":"additional","affiliation":[{"name":"Department of Biology Queen's University  Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000923\u20108"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2004.12.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2006601"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2015014"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.1370"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.01260"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010019\u20103408\u20109"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl4183"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0021-8790.2001.00534.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2021.02.008"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1139\/as\u20102018\u20100013"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.01.014"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2014.08.017"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.03.054"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074\u201002760180305"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00485"},{"key":"e_1_2_9_18_1","volume-title":"Life Sciences Group","author":"I. 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Medvecky M. Tornos J. Clessin A. Gantelet H. Gamble A. Forde T. L. &Boulinier T.(2022).Genomic characterization of a novel species ofErysipelothrixassociated with mortalities among endangered seabirds. 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