{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:48:10Z","timestamp":1753868890984,"version":"3.41.2"},"reference-count":75,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["FBIP UID 111721"],"award-info":[{"award-number":["FBIP UID 111721"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The black\u2010footed cat (<jats:italic>Felis nigripes<\/jats:italic>) is endemic to the arid regions of southern Africa. One of the world's smallest wild felids, the species occurs at low densities and is secretive and elusive, which makes ecological studies difficult. Genetic data could provide key information such as estimates on population size, sex ratios, and genetic diversity. In this study, we test if microsatellite loci can be successfully amplified from scat samples that could be noninvasively collected from the field. Using 21 blood and scat samples collected from the same individuals, we statistically tested whether nine microsatellites previously designed for use in domestic cats can be used to identify individual black\u2010footed cats. Genotypes recovered from blood and scat samples were compared to assess loss of heterozygosity, allele dropout, and false alleles resulting from DNA degradation or PCR inhibitors present in scat samples. The microsatellite markers were also used to identify individuals from scats collected in the field that were not linked to any blood samples. All nine microsatellites used in this study were amplified successfully and were polymorphic. Microsatellite loci were found to have sufficient discriminatory power to distinguish individuals and identify clones. In conclusion, these molecular markers can be used to monitor populations of wild black\u2010footed cats noninvasively. The genetic data will be able to contribute important information that may be used to guide future conservation initiatives.<\/jats:p>","DOI":"10.1002\/ece3.11315","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T06:38:38Z","timestamp":1713940718000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A method for noninvasive individual genotyping of black\u2010footed cat (<i>Felis nigripes<\/i>)"],"prefix":"10.1002","volume":"14","author":[{"given":"Vimbai I.","family":"Siziba","sequence":"first","affiliation":[{"name":"School of Life Sciences University of KwaZulu\u2010Natal  Scottsville South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle M.","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Black\u2010Footed Cat Working Group  Kimberley South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beryl","family":"Wilson","sequence":"additional","affiliation":[{"name":"McGregor Museum  Kimberley Northern Cape South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Sliwa","sequence":"additional","affiliation":[{"name":"K\u00f6lner Zoo AG (Cologne Zoo)  K\u00f6ln Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0572-369X","authenticated-orcid":false,"given":"Sandi","family":"Willows\u2010Munro","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of KwaZulu\u2010Natal  Scottsville South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01895.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-006-9154-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0962-1083.2001.01436.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10531-022-02377-x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1071\/ZO01072"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0210811","article-title":"Improving cost\u2010efficiency of fecal genotyping: New tools for elephant species","volume":"30","author":"Bourgeois S.","year":"2019","journal-title":"PLoS One"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-006-9146-5"},{"volume-title":"Travels to the interior of southern Africa","year":"1824","author":"Burchell W. 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