{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:18:31Z","timestamp":1777393111586,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,4,3]],"date-time":"2016-04-03T00:00:00Z","timestamp":1459641600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,4,3]],"date-time":"2016-04-03T00:00:00Z","timestamp":1459641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IBN\u20100316580"],"award-info":[{"award-number":["IBN\u20100316580"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R15HD076308\u201001"],"award-info":[{"award-number":["R15HD076308\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The injection of phytohaemagglutinin (<jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content>) and sampling of blood are widely used in studies of wild vertebrates to assess components of immune and endocrine function and health state and to obtain genetic material. Despite the pervasive use of these techniques in the life sciences, their potential effects on survival are rarely considered. For example, whether injection of the immunogen <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> into body parts critical for locomotion (e.g., the prepatagium, or wing web, in birds) affects survival has not been tested. Here, we test whether injection of <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> into the wing web and blood sampling from nestling house wrens affects their subsequent recruitment and survival as breeding adults. Capture\u2010mark\u2010recapture analysis on a large sample of young (<jats:italic>N\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>20,152 fledglings from 3959 broods) treated over 10\u00a0years revealed that neither <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> injection nor blood sampling affected individual survival and detection probability. Recruitment as a breeder varied among years, but this variation was not attributable to sampling effort, or the percent of all adults identified at the nest during a given year. Variation in the percent of adults identified was primarily attributable to the effect of nest depredation on our ability to capture nesting pairs. Our results indicating lack of an effect of blood sampling and immune stimulation on survival are encouraging, but we recommend further work to assess the potential negative effects of all commonly used techniques on the survival of study subjects in the wild, including the potential costs associated with mounting various immunological responses.<\/jats:p>","DOI":"10.1002\/ece3.2112","type":"journal-article","created":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T01:39:28Z","timestamp":1459733968000},"page":"3107-3114","source":"Crossref","is-referenced-by-count":7,"title":["No effect of blood sampling or phytohaemagglutinin injection on postfledging survival in a wild songbird"],"prefix":"10.1002","volume":"6","author":[{"given":"Emerson Keith","family":"Bowers","sequence":"first","affiliation":[{"name":"Behavior, Ecology, Evolution, and Systematics Section School of Biological Sciences Illinois State University Normal Illinois 61790\u20104120"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott K.","family":"Sakaluk","sequence":"additional","affiliation":[{"name":"Behavior, Ecology, Evolution, and Systematics Section School of Biological Sciences Illinois State University Normal Illinois 61790\u20104120"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles F.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Behavior, Ecology, Evolution, and Systematics Section School of Biological Sciences Illinois State University Normal Illinois 61790\u20104120"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.049528"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00300-010-0888-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-7590.2007.04939.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1990"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1999.0660"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/670244"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1890\/14-0418.1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/681017"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1525\/auk.2009.09048"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00265-003-0682-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2005.00910.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2656.2011.01813.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/auk\/105.3.480"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/649894"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17715"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2173\/bna.380"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3161\/000164510X516047"},{"key":"e_1_2_7_19_1","first-page":"534","article-title":"Blood sampling in juvenile buff\u2010breasted sandpipers: movement, mass change and survival","volume":"65","author":"Lancot R. B.","year":"1994","journal-title":"J. Field Ornithol."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2656.2003.00734.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2010.01783.x"},{"key":"e_1_2_7_22_1","first-page":"595","article-title":"Effects of blood sampling in great tits","volume":"69","author":"Lubjuhn T.","year":"1998","journal-title":"J. Field Ornithol."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2002.2185"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2006.01094.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.1999.1127"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0908-8857.2005.03413.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0021-8790.2001.00533.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1139\/Z10-020"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1525\/auk.2011.10289"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10336-003-0009-2"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0908-8857.2008.04295.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.2307\/1382950"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2307\/5035"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2010.01711.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-048X.2011.05860.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/00063659909477239"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.2307\/2265623"}],"container-title":["Ecology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fece3.2112","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.2112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ece3.2112","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.2112","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.2112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T04:44:17Z","timestamp":1696567457000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ece3.2112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,3]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/ece3.2112"],"URL":"https:\/\/doi.org\/10.1002\/ece3.2112","archive":["Portico"],"relation":{},"ISSN":["2045-7758","2045-7758"],"issn-type":[{"value":"2045-7758","type":"print"},{"value":"2045-7758","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,3]]}}}