{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:14:10Z","timestamp":1790705650086,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001732","name":"Danmarks Grundforskningsfond","doi-asserted-by":"publisher","award":["DNRF94"],"award-info":[{"award-number":["DNRF94"]}],"id":[{"id":"10.13039\/501100001732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003554","name":"Lundbeckfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent ancient <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">aDNA<\/jats:styled-content>) studies of human pathogens have provided invaluable insights into their evolutionary history and prevalence in space and time. Most of these studies were based on <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extracted from teeth or postcranial bones. In contrast, no pathogen <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> has been reported from the petrous bone which has become the most desired skeletal element in ancient <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> research due to its high endogenous <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> content. To compare the potential for pathogenic <jats:styled-content style=\"fixed-case\">aDNA<\/jats:styled-content> retrieval from teeth and petrous bones, we sampled these elements from five ancient skeletons, previously shown to be carrying <jats:italic>Yersinia pestis<\/jats:italic>. Based on shotgun sequencing data, four of these five plague victims showed clearly detectable levels of <jats:italic>Y.\u00a0pestis <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in the teeth, whereas all the petrous bones failed to produce <jats:italic>Y.\u00a0pestis <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> above baseline levels. A broader comparative metagenomic analysis of teeth and petrous bones from 10 historical skeletons corroborated these results, showing a much higher microbial diversity in teeth than petrous bones, including pathogenic and oral microbial taxa. Our results imply that although petrous bones are highly valuable for ancient genomic analyses as an excellent source of endogenous <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>, the metagenomic potential of these dense skeletal elements is highly limited. This trade\u2010off must be considered when designing the sampling strategy for an <jats:styled-content style=\"fixed-case\">aDNA<\/jats:styled-content> project.<\/jats:p>","DOI":"10.1002\/ece3.3924","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T04:56:40Z","timestamp":1519707400000},"page":"3534-3542","source":"Crossref","is-referenced-by-count":57,"title":["Ancient pathogen <scp>DNA<\/scp> in human teeth and petrous bones"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2576-2429","authenticated-orcid":false,"given":"Ashot","family":"Margaryan","sequence":"first","affiliation":[{"name":"Centre for GeoGenetics Natural History Museum of Denmark University of Copenhagen  Copenhagen Denmark"},{"name":"Institute of Molecular Biology National Academy of Sciences  Yerevan Armenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik 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Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liivi","family":"Varul","sequence":"additional","affiliation":[{"name":"School of Humanities Tallinn University  Tallinn Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehti","family":"Saag","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Biology Institute of Molecular and Cell Biology University of Tartu  Tartu Estonia"},{"name":"Estonian Biocentre Institute of Genomics University of Tartu  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Lynnerup","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Section of Forensic Pathology University of Copenhagen  Copenhagen East Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eske","family":"Willerslev","sequence":"additional","affiliation":[{"name":"Centre for GeoGenetics Natural History Museum of Denmark University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Zoology University of Cambridge  Cambridge UK"},{"name":"Wellcome Trust Sanger Institute Hinxton  Cambridgeshire CB10 1SA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten E.","family":"Allentoft","sequence":"additional","affiliation":[{"name":"Centre for GeoGenetics Natural History Museum of Denmark University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14507"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.08.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348946607500402"},{"key":"e_1_2_8_5_1","volume-title":"The genus aeromonas","author":"Austin 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