{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:14:09Z","timestamp":1764656049677,"version":"3.40.5"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many bird species are sexually monomorphic and cannot be sexed based on phenotypic traits. Rapid sex determination is often a necessary component of avian studies focusing on behavior, ecology, evolution, and conservation. While PCR\u2010based methods are the most common technique for molecularly sexing birds in the laboratory, a simpler, faster, and cheaper method has emerged, which can be used in the laboratory, but importantly also in the field. Herein, we used loop\u2010mediated isothermal amplification (LAMP) for rapid sex determination of blood samples from juvenile European blackcaps, <jats:italic>Sylvia atricapilla<\/jats:italic>, sampled in the wild. We designed LAMP primers unique to <jats:italic>S.\u00a0atricapilla<\/jats:italic> based on the sex chromosome\u2010specific gene, chromo\u2010helicase\u2010DNA\u2010binding protein (CHD), optimized the primers for laboratory and field application, and then used them to test a subset of wild\u2010caught juvenile blackcaps of unknown gender at the time of capture. Sex determination results were fast and accurate. The advantages of this technique are that it allows researchers to identify the sex of individual birds within hours of sampling and eliminates the need for direct access to a laboratory if implemented at a remote field site. This work adds to the increasing list of available LAMP primers for different bird species and is a new addition within the Passeriformes order.<\/jats:p>","DOI":"10.1002\/ece3.5168","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T13:50:50Z","timestamp":1555336250000},"page":"5849-5858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Rapid sex determination of a wild passerine species using loop\u2010mediated isothermal amplification (LAMP)"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1776-9487","authenticated-orcid":false,"given":"Hanna R.","family":"Koch","sequence":"first","affiliation":[{"name":"Max Planck Research Group Behavioral Genomics Max Planck Institute for Evolutionary Biology Pl\u00f6n Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elke","family":"Blohm\u2010Sievers","sequence":"additional","affiliation":[{"name":"Max Planck Research Group Behavioral Genomics Max Planck Institute for Evolutionary Biology Pl\u00f6n Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8372-8560","authenticated-orcid":false,"given":"Miriam","family":"Liedvogel","sequence":"additional","affiliation":[{"name":"Max Planck Research Group Behavioral Genomics Max Planck Institute for Evolutionary Biology Pl\u00f6n Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04054"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/360668a0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12540"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12732"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.05.034"},{"volume-title":"The Birds of the Western Olearctic","year":"1992","author":"Cramp S.","key":"e_1_2_9_7_1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1996.0239"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5347(97)01061-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2307\/3677252"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-016-2618-z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.034025-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.1998.00389.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00266-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/375454a0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.12-0208"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00121451"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1557-9263.2006.00033.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00172133"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4cc00540f"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03019.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1681-3"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.20.339"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2307\/4089527"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.11.025"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3906\/vet-1410-84"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075821"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2009.08.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12548"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN01897E"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5921"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.04.015"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39090"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2008.05.001"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.593"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1554\/03-731"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910361107"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-010-0265-1"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.57"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.21010"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20046048"}],"container-title":["Ecology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fece3.5168","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.5168","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ece3.5168","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.5168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T15:33:13Z","timestamp":1694359993000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ece3.5168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,15]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/ece3.5168"],"URL":"https:\/\/doi.org\/10.1002\/ece3.5168","archive":["Portico"],"relation":{},"ISSN":["2045-7758","2045-7758"],"issn-type":[{"type":"print","value":"2045-7758"},{"type":"electronic","value":"2045-7758"}],"subject":[],"published":{"date-parts":[[2019,4,15]]},"assertion":[{"value":"2018-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}