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Earlier studies have found that\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    is commonly detected in\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0albopictus<\/jats:italic>\n                    but rarely detected in\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    . In this study, we used a two\u2010step PCR assay to detect\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    in wild\u2010collected samples of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    . The PCR products were sequenced to validate amplicons and identify\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strains. A loop\u2010mediated isothermal amplification (LAMP) assay was developed and used for detecting\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    in selected mosquito specimens as well. We found\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    in 85\/148 (57.4%) wild\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    specimens from various cities in New Mexico, and in 2\/46 (4.3%) from St. Augustine, Florida.\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    was not detected\u00a0in 94 samples of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    from Deer Park, Harris County, Texas.\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    detected in\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    from both New Mexico and Florida was the\n                    <jats:italic>w<\/jats:italic>\n                    AlbB strain of\n                    <jats:italic>Wolbachia pipientis<\/jats:italic>\n                    . A\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    \u2010positive colony of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    was established from pupae collected in Las Cruces, New Mexico, in 2018. The infected females of this strain transmitted\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    to their progeny when crossed with males of Rockefeller strain of\n                    <jats:italic>Ae.\u00a0aegypti<\/jats:italic>\n                    , which does not carry\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    . In contrast, none of the progeny of Las Cruces males mated to Rockefeller females were infected with\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/ece3.5198","type":"journal-article","created":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T05:27:38Z","timestamp":1556256458000},"page":"6148-6156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["<i>Wolbachia pipientis<\/i>\n                    occurs in\n                    <i>Aedes aegypti<\/i>\n                    populations in New Mexico and Florida, USA"],"prefix":"10.1002","volume":"9","author":[{"given":"Aditi","family":"Kulkarni","sequence":"first","affiliation":[{"name":"Biology Department New Mexico State University Las Cruces New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqin","family":"Yu","sequence":"additional","affiliation":[{"name":"Biology Department New Mexico State University Las Cruces New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Biology Department New Mexico State University Las Cruces New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepcion","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Biology Department New Mexico State University Las Cruces New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajit K.","family":"Karna","sequence":"additional","affiliation":[{"name":"Biology Department New Mexico State University Las Cruces New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalli J. 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