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However, the DNA of many species cannot be processed immediately at the sampling site due to the remoteness of the location, necessitating tissue preservation that may affect DNA fragment size. This study aimed to identify the most suitable combination of four tissue preservation approaches and six DNA extraction methods to ensure high molecular weight DNA. A single <jats:italic>Peltodoris atromaculata<\/jats:italic> (Nudibranchia) specimen was sliced into \u223c30\u2009mg sub\u2010samples, ensuring consistency across 24 preservation\u2010extraction combinations processed in triplicates. Samples were either stored at 4\u00b0C, dried at room temperature, flash\u2010frozen in liquid nitrogen, or preserved in ethanol and stored at \u221220\u00b0C. Afterward, they were processed using five commercially available kits specific for high molecular weight DNA extraction, as well as a custom DNA extraction protocol. Three aspects of DNA quality were evaluated: total yield, fragment size distribution, and availability of DNA for amplification. Most preservation\u2010extraction combinations yielded optimal results in only some of the three DNA quality aspects. We identified six combinations suitable for long\u2010read sequencing: a custom CTAB\u2010based extraction protocol applied to frozen samples, Wizard (Promega) and Nanobind (PacBio) kits for both frozen and ethanol\u2010preserved samples, and the ethanol preservation paired with Monarch (NEB) kits. The suitability of the six selected combinations was confirmed by PacBio sequencing, producing a total yield of 3.6 Gbp (3.2x estimated genome coverage). The results indicate that the success of high molecular weight DNA extractions is influenced by preservation methods. Although tested on nudibranchs, these findings are highly useful for genomic studies of other organisms, which may need to be preserved in remote locations before being transported to the laboratory for processing.<\/jats:p>","DOI":"10.1002\/ece3.71262","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T07:45:51Z","timestamp":1744703151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Combination of Sample Preservation Approaches and <scp>DNA<\/scp> Extraction Methods for Long\u2010Read Sequencing of Nudibranchs' Genomes"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4097-5816","authenticated-orcid":false,"given":"In\u00e9s","family":"Alberola\u2010Mora","sequence":"first","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio) University of Valencia and Spanish National Research Council (CSIC)  Valencia 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