{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T19:28:01Z","timestamp":1773170881504,"version":"3.50.1"},"reference-count":70,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160127"],"award-info":[{"award-number":["32160127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360670"],"award-info":[{"award-number":["32360670"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The insect olfactory system relies on a sophisticated repertoire of olfactory\u2010related proteins to enable precise odor detection and subsequent signal transduction. Their expression and regulation are crucial for mediating communication between themselves and environments.\n                    <jats:italic>Lytta sifanica<\/jats:italic>\n                    (Coleoptera: Meloidae), a species of significant economic importance, represents a notable subject for olfactory studies, particularly as it is renowned for producing the medically relevant toxin cantharidin. However, the molecular basis of olfactory sensation in the Blister Beetle\n                    <jats:italic>Lytta sifanica<\/jats:italic>\n                    has not yet been characterized. In this study, the transcriptomes of adult\n                    <jats:italic>L. sifanica<\/jats:italic>\n                    antennae were sequenced and analyzed. A total of 70 chemosensory genes, including 17 odorant binding proteins (OBPs), 5 chemosensory proteins (CSPs), 13 gustatory receptors (GRs), 17 odorant receptors (ORs), 13 ionotropic receptors (IRs) and 5 sensory neuron membrane proteins (SNMPs) were identified based on sequence homology analysis and phylogenetic reconstruction. The expression profiles of all candidate genes were confirmed by RT\u2010PCR across multiple tissues, including antennae, head, mouthparts, pronotum, foreleg tarsus, abdomen skin, and wings. The results revealed that olfactory\u2010related protein families were broadly expressed in the tested tissues, though with distinct tissue\u2010specific patterns among different gene families. Among the identified chemosensory genes, eleven members are predominantly expressed in the antennae (\n                    <jats:italic>Lsif_OBP1\/2\/19d, Lsif_OR2\/20\/49b, Lsif_IR2a, Lsif_GR7\/127, Lsif_CSP1<\/jats:italic>\n                    , and\n                    <jats:italic>Lsif_SNMP2\/4<\/jats:italic>\n                    ). In contrast, twenty\u2010two members were exclusively expressed in the mouthparts (\n                    <jats:italic>Lsif_OBP70\/56d2, Lsif_GR12a\/21\/28a\/68a<\/jats:italic>\n                    ), foreleg tarsus (\n                    <jats:italic>Lsif_OR6\/67c, Lsif_GR12,<\/jats:italic>\n                    and\n                    <jats:italic>Lsif_OBP2<\/jats:italic>\n                    ) and were abundant in the non\u2010olfactory tissues head (\n                    <jats:italic>Lsif_OBP99a, Lsif_OR9a\/49b, Lsif_IR25a\/56e,<\/jats:italic>\n                    and\n                    <jats:italic>Lsif_CSP6<\/jats:italic>\n                    ), pronotum (\n                    <jats:italic>Lsif_OBP5\/C20, Lsif_ORco, Lsif_OR13<\/jats:italic>\n                    , and\n                    <jats:italic>Lsif_IR7<\/jats:italic>\n                    ), abdomen skin (\n                    <jats:italic>Lsif_SNMP5<\/jats:italic>\n                    ), suggesting their various functions in the olfactory system of\n                    <jats:italic>L. sifanica<\/jats:italic>\n                    . This research offers an extensive resource for investigating the chemoreception mechanism in beetle\n                    <jats:italic>L. sifanica<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/ece3.72634","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:10:31Z","timestamp":1765354231000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemosensory System Decoding: Transcriptome\u2010Wide Identification and Expression Profiling of Olfactory Genes in\n                    <i>Lytta sifanica<\/i>"],"prefix":"10.1002","volume":"15","author":[{"given":"Feng","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Life Science Northwest Normal University  Lanzhou 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