{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:28:00Z","timestamp":1787030880906,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004593","name":"Universidad Aut\u00f3noma de Madrid","doi-asserted-by":"publisher","award":["PIPF\u20102022\/SAL\u2010GL\u201026101"],"award-info":[{"award-number":["PIPF\u20102022\/SAL\u2010GL\u201026101"]}],"id":[{"id":"10.13039\/501100004593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Formalin, ethanol, and\n                    <jats:italic>RNAlater<\/jats:italic>\n                    are the most commonly used fixatives for morphological and molecular studies. Formalin is preferred for preserving tissue morphology, whereas ethanol and\n                    <jats:italic>RNAlater<\/jats:italic>\n                    are used to obtain high\u2010quality nucleic acids for molecular analyses, including emerging \u2010omics techniques. Over the past few years, the study of non\u2010model organisms has gained attention, but the lack of laboratory cultures for many species requires collecting and fixing the animals directly in the field. Very often, just a few specimens are secured, limiting the possibility of using multiple fixatives for parallel analyses. A single fixative that preserves both morphology and molecules while being easy to handle in the field would therefore be highly valuable. KINFix, a non\u2010toxic alcohol\u2010based fixative, was developed to preserve histology, proteins, and nucleic acids simultaneously, enabling both morphological and molecular analyses with the same sample. Here, we evaluate the suitability of KINFix for electron microscopy, RNA preservation, and cell dissociation for single\u2010cell RNA sequencing (scRNA\u2010seq) experiments, using four invertebrate species from different spiralian phyla. Our results demonstrate that KINFix maintains RNA integrity for over 3\u2009months, similarly to other standard fixatives, but also preserves morphology and cellular integrity even after cell dissociation, suggesting its suitability for scRNA\u2010seq applications. While fixation conditions may require optimization for different species and tissues, our findings highlight KINFix as a cost\u2010effective, versatile, and valuable fixative that enables a wide range of morphological and molecular studies in non\u2010model invertebrates. KINFix is particularly useful for field\u2010based research where sample availability and preservation logistics are especially challenging.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73006","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:12:13Z","timestamp":1770743533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Field\u2010Friendly, Non\u2010Toxic Fixative for Integrated Morphological and Molecular Research in Non\u2010Model Invertebrates"],"prefix":"10.1002","volume":"16","author":[{"given":"Irene","family":"del Olmo","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Moreno\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Universidad Aut\u00f3noma de 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