{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T05:47:17Z","timestamp":1782107237607,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370441"],"award-info":[{"award-number":["32370441"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Body color variation is widespread among animals, and the melanocortin 1 receptor (MC1R) acts as a vital switch gene in regulating pigmentation. A small number of amino acid substitutions, or even a single one, in MC1R are known to influence the body coloration of animals. To investigate the associations between MC1R variants and body color variation in melanistic and non\u2010melanistic populations of a toad\u2010headed lizard,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. versicolor<\/jats:italic>\n                    <\/jats:styled-content>\n                    , we obtained the complete coding sequences of the\n                    <jats:italic>MC1R<\/jats:italic>\n                    gene. Sequence analysis identified two amino acid substitutions\u2014V165I and V237I, located in transmembrane domains 4 (TM4) and 6 (TM6), respectively\u2014that were significantly associated with color variation in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. versicolor<\/jats:italic>\n                    <\/jats:styled-content>\n                    . In silico structural analysis incorporating these two substitutions demonstrated that the melanistic MC1R exhibited stronger binding affinity for \u03b1\u2010MSH, as characterized by a smaller binding surface area and volume, a greater number of hydrogen bonds, more favorable binding free energy, a higher binding constant, and a lower dissociation constant. These changes may ultimately enhance melanin synthesis in melanistic populations. Our results provide insights into how amino acid substitutions affect receptor\u2013ligand\u2010binding capacity by altering the pocket size of MC1R in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. versicolor<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/ece3.73481","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T05:12:22Z","timestamp":1777007542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Associations of\n                    <scp>MC1R<\/scp>\n                    Substitutions With Body Color Variation in a Desert Lizard"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7094-2832","authenticated-orcid":false,"given":"Haojie","family":"Tong","sequence":"first","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjin","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangyue","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1207-6254","authenticated-orcid":false,"given":"Kaixin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2401-6077","authenticated-orcid":false,"given":"Richard P.","family":"Brown","sequence":"additional","affiliation":[{"name":"School of Biological and Environmental Sciences Liverpool John Moores University  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1001-2158","authenticated-orcid":false,"given":"Yuanting","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou P. R. 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