{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:24:40Z","timestamp":1792002280052,"version":"4.4.0"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Ranunculus<\/jats:italic>\n                    L., the largest genus of Ranunculaceae, exhibits remarkable ecological diversity, yet genomic resources for this genus remain limited. Here we report the complete chloroplast (cp) genomes of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ranunculus arvensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    L. and\n                    <jats:italic>Ranunculus laetus<\/jats:italic>\n                    Wall. ex Hook.f. &amp; Thomson. The\n                    <jats:italic>R. laetus<\/jats:italic>\n                    assembly is the first cp genome reported for this species, whereas the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. arvensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    assembly, generated from a Pakistani population, provides an independent accession that complements the recently released GenBank record PV621859 from China. Both genomes were analyzed comparatively against 22 previously published\n                    <jats:italic>Ranunculus<\/jats:italic>\n                    cp genomes. The cp genomes ranged from 154,474 to 158,314 bp in length and displayed a typical quadripartite structure. All analyzed species harbored 112 unique genes, comprising 78 protein\u2010coding genes, 30 transfer RNA genes, and four ribosomal RNA genes. The cp genomes were highly conserved in terms of gene order, guanine\u2013cytosine content, codon usage, amino acid composition, and simple sequence repeats. Phylogeny\u2010aware selection analysis models (BUSTED, MEME, FUBAR) revealed that, despite overall purifying selection at the gene level, codon\u2010based FUBAR detected pervasive positive selection across ~34.6% of plastid genes;\n                    <jats:italic>matK<\/jats:italic>\n                    and\n                    <jats:italic>ycf1<\/jats:italic>\n                    emerged as the most prominent hotspots for both episodic and pervasive adaptation. Nucleotide diversity analyses revealed several highly variable regions, notably\n                    <jats:italic>rpl32\u2013ndhF<\/jats:italic>\n                    ,\n                    <jats:italic>ycf1<\/jats:italic>\n                    , and\n                    <jats:italic>ndhF<\/jats:italic>\n                    . Phylogenetic analyses based on complete cp genome sequences and complementary single\u2010gene trees resolved two major clades corresponding to biogeographic patterns: Clade I comprised predominantly Eurasian and East Asian taxa, whereas Clade II comprised a transcontinental assemblage of East Asian, North American, and Himalayan species. The\n                    <jats:italic>R. laetus<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R. arvensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    were grouped in Clade II. Overall, these results elucidate the conserved genomic architecture, evolutionary dynamics, and phylogenetic relationships of\n                    <jats:italic>Ranunculus<\/jats:italic>\n                    cp genomes and provide valuable genomic resources for future studies on phylogeny, taxonomy, DNA barcoding, and population genetics within Ranunculaceae.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73559","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:25:52Z","timestamp":1777515952000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Complete Chloroplast Genomes of\n                    <i>Ranunculus arvensis<\/i>\n                    and\n                    <i>Ranunculus laetus<\/i>\n                    : Comparative Analysis and Phylogenetic Insights"],"prefix":"10.1002","volume":"16","author":[{"given":"Hui","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1628-8478","authenticated-orcid":false,"family":"Abdullah","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayed Afzal","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National University of Medical Sciences  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liya","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional Chinese Medicine  Tianjin China"},{"name":"Haihe Laboratory of Modern Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao\u2010di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing P. 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