{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:07:02Z","timestamp":1784610422081,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013997","name":"Sasakawa Peace Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013997","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Modern, integrative biodiversity research requires methods capable of bridging the gap between detailed morphological observations and the scalability of DNA sequencing. Integrative approaches like megabarcoding generate DNA sequences, while morphological functional data and abundance data are retained. For small, transparent, and highly abundant animals like meiofauna, the necessity to sort the specimens from organic matter and sediment forms a major bottleneck, as the use of stains required for enhanced manual or automated specimen detection can inhibit downstream molecular workflows. To address this, we tested the compatibility of phloxine B staining with DNA sequencing to facilitate simultaneous morphological and molecular specimen processing. Meiofauna preserved in ethanol, propylene glycol, or a DMSO\/EDTA\/saturated NaCl solution (DESS) was incubated with phloxine B for 30\u2009min or 24\u2009h. Specimens were manually isolated, the V1\u2013V2 region of the 18S rDNA gene was sequenced, and success was quantified across major meiofaunal phyla. Nematodes, copepods, and annelids consistently showed high sequencing success irrespective of the preservative or staining incubation time. Platyhelminths showed lower success, likely due to misidentification or primer limitations rather than dye inhibition. Overall, these findings demonstrate that phloxine B is compatible with downstream DNA amplification and sequencing, enabling efficient integration of morphological and molecular data. The approach offers high potential for broader application in other microscopic taxa, supporting the way to comprehensive, high\u2010throughput biodiversity assessments or ecological monitoring.<\/jats:p>","DOI":"10.1002\/ece3.73597","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:51:17Z","timestamp":1777607477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phloxine B Staining is Compatible With High\u2010Throughput\n                    <scp>DNA<\/scp>\n                    Barcoding of Meiofauna"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3068-550X","authenticated-orcid":false,"given":"Romy Lisanne","family":"Gielings","sequence":"first","affiliation":[{"name":"Naturalis Biodiversity Center  Leiden the Netherlands"},{"name":"University of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics (IBED)  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Foulon","sequence":"additional","affiliation":[{"name":"Univ. Brest, Ifremer, BEEP  Plouzan\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem","family":"Renema","sequence":"additional","affiliation":[{"name":"Naturalis Biodiversity Center  Leiden the Netherlands"},{"name":"University of Amsterdam, Institute for Biodiversity and Ecosystem Dynamics (IBED)  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-7522","authenticated-orcid":false,"given":"Jan\u2010Niklas","family":"Macher","sequence":"additional","affiliation":[{"name":"Naturalis Biodiversity Center  Leiden the Netherlands"},{"name":"Leiden University, Institute of Environmental Sciences (CML), Department of Environmental Biology  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2012.07.013"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0077-7579(86)90005-0"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.17504\/protocols.io.587g9zn"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/24.11_ts.1324"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/32160"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps09870"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margen.2015.04.010"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/syen.12624"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2023.02.012"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033801"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0309463"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2018.06.013"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jembe.2014.06.016"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2012.10.516"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1095"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/lom3.10690"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cla.12453"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2021.730063"},{"key":"e_1_2_12_20_1","first-page":"63","volume-title":"Sampling and Processing Meiofauna. 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