{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:06:21Z","timestamp":1784610381702,"version":"3.55.0"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["lzujbky\u20102024\u2010ey01"],"award-info":[{"award-number":["lzujbky\u20102024\u2010ey01"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The grass genus\n                    <jats:italic>Orinus<\/jats:italic>\n                    , endemic to the Qinghai\u2010Tibet Plateau (QTP), has long been taxonomically controversial. Recent integrative studies combining morphological and DNA barcoding proposed revising the genus from six to three species; however, this hypothesis lacks validation from genome\u2010wide data, and the evolutionary history of the putative species remains poorly resolved. Here, we conducted whole\u2010genome resequencing of 126 individuals from 40 populations representing three putative species of\n                    <jats:italic>Orinus<\/jats:italic>\n                    \u2014\n                    <jats:italic>Orinus thoroldii<\/jats:italic>\n                    ,\n                    <jats:italic>Orinus kokonorica<\/jats:italic>\n                    , and\n                    <jats:italic>Orinus intermedius<\/jats:italic>\n                    \u2014and performed population genomic and demographic analyzes. Genome\u2010wide evidence robustly supports the three\u2010species delimitation and reveals pronounced genetic differentiation among lineages. We further detected both historical and recent hybridization between\n                    <jats:italic>O. kokonorica<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>O. intermedius<\/jats:italic>\n                    <\/jats:styled-content>\n                    , indicating incomplete reproductive isolation. Demographic inference suggests that divergence among lineages occurred during Quaternary climatic oscillations, when repeated climate fluctuations, together with the complex topography of the QTP, likely promoted lineage divergence while allowing persistent and asymmetric gene flow, particularly during early stages of divergence. Across species pairs, we consistently identified genomic islands of divergence characterized by elevated absolute nucleotide divergence (\n                    <jats:italic>D<\/jats:italic>\n                    <jats:sub>xy<\/jats:sub>\n                    ). Genes located within these regions show signatures of positive selection and are enriched in functions related to environmental adaptation, suggesting a role of natural selection in maintaining species boundaries. Together, our results provide robust genomic support for the revised taxonomy of\n                    <jats:italic>Orinus<\/jats:italic>\n                    and offer new insights into how Quaternary environmental dynamics have shaped speciation and genomic divergence in a montane grass lineage.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73641","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T06:45:09Z","timestamp":1778481909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Insights Into Species Divergence and Adaptive Evolution in the Grass Genus\n                    <i>Orinus<\/i>\n                    on the Qinghai\u2010Tibet Plateau"],"prefix":"10.1002","volume":"16","author":[{"given":"Ai","family":"Liu","sequence":"first","affiliation":[{"name":"College of Geology and Jewelry Lanzhou Resources &amp; Environment Voc\u2010Tech University  Lanzhou China"},{"name":"Yellow River Basin Ecotope Integration of Industry and Education Research Institute Lanzhou Resources &amp; Environment Voc\u2010Tech University  Lanzhou China"},{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Geology and Jewelry Lanzhou Resources &amp; Environment Voc\u2010Tech University  Lanzhou China"},{"name":"Yellow River Basin Ecotope Integration of Industry and Education Research Institute Lanzhou Resources &amp; Environment Voc\u2010Tech University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunjing","family":"Qu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai\u2010Tibet Plateau in Qinghai Province Qinghai Normal University  Xining China"},{"name":"Academy of Plateau Science and Sustainability Qinghai Normal University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Su","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai\u2010Tibet Plateau in Qinghai Province Qinghai Normal University  Xining China"},{"name":"Academy of Plateau Science and Sustainability Qinghai Normal University  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangpeng","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3318-7794","authenticated-orcid":false,"given":"Hongyin","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Herbage Improvement and Grassland Agro\u2010Ecosystems, College of Ecology Lanzhou University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1420-9101.2012.02599.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.094052.109"},{"key":"e_1_2_10_4_1","volume-title":"Darwin and Modern Science","author":"Bateson W.","year":"1909"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/521987"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.135269"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-5347(02)02540-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr330"},{"key":"e_1_2_10_10_1","volume-title":"On the Origin of Species","author":"Darwin C.","year":"1859"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.806"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11584"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab468"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.03.009"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14290"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16925"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.212522.116"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-021-02415-8"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/35016000"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2003.1388"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04004"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16622"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2025711118"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/116.1.153"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/17550874.2021.2012849"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13652"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16226"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010014\u20100356\u20104"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1461\u20100248.2012.01746.x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1195755"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1502-3885.2005.tb01008.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10231"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.176214"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jse.12665"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jse.12094"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1713288114"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.159426.113"},{"key":"e_1_2_10_40_1","volume-title":"Systematics and the Origin of Species","author":"Mayr E.","year":"1942"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000939107"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2011.09.002"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2008.03946.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020190"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002967"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1086\/519795"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-05620-5"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-015-0445-7"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jbi.13415"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14221"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.17822"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.301"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3644"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.110308.120317"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcv062"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12599"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.15388"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.133"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1420-9101.2001.00335.x"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17956"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1190371"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty875"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-6182(02)00054-X"}],"container-title":["Ecology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.73641","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ece3.73641","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.73641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:39:59Z","timestamp":1784608799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ece3.73641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":62,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/ece3.73641"],"URL":"https:\/\/doi.org\/10.1002\/ece3.73641","archive":["Portico"],"relation":{},"ISSN":["2045-7758","2045-7758"],"issn-type":[{"value":"2045-7758","type":"print"},{"value":"2045-7758","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2026-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e73641"}}