{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:05:52Z","timestamp":1784261152547,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The complete chloroplast genome of\n                    <jats:italic>Caltha scaposa<\/jats:italic>\n                    , a high\u2010altitude wetland species native to the Himalayas and Hengduan Mountains, was assembled and characterized to clarify its phylogenetic position and explore plastome structural variation within the tribe Anemoneae. The circular genome is 160,219,155,293\u2009bp in length and exhibits a typical quadripartite structure, including a LSC (large single copy) region of 79,994\u2009bp, an SSC (small single copy) region of 17,075\u2009bp, and a pair of IRs (inverted repeats) of 26,410\u2009bp each. The genome encodes 132 genes, comprising 87 protein\u2010coding genes, 37 tRNA genes, and four rRNA genes. A total of 54 SSRs (simple sequence repeats) were identified, predominantly mononucleotide A\/T repeats. Comparative analysis with nine related species from the genera\n                    <jats:italic>Anemone<\/jats:italic>\n                    and\n                    <jats:italic>Pulsatilla<\/jats:italic>\n                    revealed significant variation in IR boundaries.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Caltha scaposa<\/jats:italic>\n                    <\/jats:styled-content>\n                    possesses the shortest IR regions, while all examined\n                    <jats:italic>Pulsatilla<\/jats:italic>\n                    species exhibit expanded IRs, resulting in structural rearrangements. Nucleotide diversity analysis indicated that intergenic spacers, especially\n                    <jats:italic>ndhE<\/jats:italic>\n                    \u2010\n                    <jats:italic>ndhG<\/jats:italic>\n                    (Pi\u2009=\u20090.1195), are highly variable, whereas tRNA genes are extremely conserved. Codon usage analysis revealed a bias toward A\/T\u2010ending codons. Phylogenomic reconstruction based on 40 complete plastomes using maximum likelihood and Bayesian methods robustly placed\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. scaposa<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a sister lineage to the clade comprising\n                    <jats:italic>Anemone<\/jats:italic>\n                    and\n                    <jats:italic>Pulsatilla<\/jats:italic>\n                    within tribe Anemoneae. These findings clarify the systematic position of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. scaposa<\/jats:italic>\n                    <\/jats:styled-content>\n                    , highlight dynamic IR evolution in Anemoneae, and provide valuable genomic resources for future evolutionary and population genetic studies in Ranunculaceae.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73728","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T01:01:16Z","timestamp":1779930076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Complete Chloroplast Genomes of\n                    <i>Caltha scaposa<\/i>\n                    for Identification and Phylogenetic Analysis"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9517-8317","authenticated-orcid":false,"given":"Le","family":"Chen","sequence":"first","affiliation":[{"name":"Beijing Botanical Garden Management Office Beijing Floriculture Engineering Technology Research Center, Key Laboratory of National Forestry and Grassland Administration on Plant Ex Situ Conservation  Beijing China"},{"name":"College of Landscape Architecture Beijing Forestry University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongming","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Human Settlements North China University of Water Resources and Electric Power  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihang","family":"Chen","sequence":"additional","affiliation":[{"name":"GMU\u2010GIBH Joint School of Life Sciences, The Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory for Cell Fate Regulation and Diseases Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Landscape Architecture Beijing Forestry University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai","family":"Gao","sequence":"additional","affiliation":[{"name":"Beijing Botanical Garden Management Office Beijing Floriculture Engineering Technology Research Center, Key Laboratory of National Forestry and Grassland Administration on Plant Ex Situ Conservation  Beijing China"},{"name":"College of Landscape Architecture Beijing Forestry University  Beijing 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