{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:05:53Z","timestamp":1784261153938,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007665","name":"Save Our Seas Foundation","doi-asserted-by":"publisher","award":["459"],"award-info":[{"award-number":["459"]}],"id":[{"id":"10.13039\/501100007665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Environmental DNA (eDNA) has emerged as a powerful tool to support the conservation of threatened species. The sawfishes are one of the most imperiled vertebrate groups, with all five species classified as Critically Endangered on the IUCN Red List of Threatened Species. Owing to their toothy rostra, sawfishes are easily entangled in a wide range of fishing gear, with mortalities in fisheries and loss of habitat driving global declines. The Largetooth Sawfish,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pristis pristis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , historically had a circumtropical range, but has experienced severe declines in geographic range and abundance. Viable populations are thought to be confined to northern Australia, but a handful of other nations may harbor remnant populations. To identify promising locations where populations may persist, a novel species\u2010specific Droplet Digital PCR (ddPCR) assay was designed capable of detecting a single copy of DNA (Limit of Detection 0.07 copies\/\u03bcL), with in silico and in\u00a0vitro validation experiments indicating functionality across the species' historical range. Water samples were then collected in 2022 at 99 sites in the Eastern Tropical Pacific from the Gulf of Chiriqu\u00ed (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u200948) and Dari\u00e9n (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u200918) regions of Panama, and the Choc\u00f3 region (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u200933) of Colombia, but\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. pristis<\/jats:italic>\n                    <\/jats:styled-content>\n                    DNA was not detected at any sampling sites. Habitat Suitability Index modeling revealed that sampling sites were located within, or in proximity to, basins with highly suitable habitats for sawfish. Detecting rare species in remote data\u2010poor regions presents major challenges, and additional spatially and temporally comprehensive eDNA surveys using this novel ddPCR eDNA assay are recommended. These surveys should focus on locations where the presence of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. pristis<\/jats:italic>\n                    <\/jats:styled-content>\n                    remains uncertain to identify priority locations for global conservation efforts.\n                  <\/jats:p>","DOI":"10.1002\/ece3.73733","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:23:59Z","timestamp":1780442639000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One Assay to Rule Them All: Development of a Global Environmental\n                    <scp>DNA<\/scp>\n                    Tool to Support Range\u2010Wide Surveys of an Imperiled Sawfish and Its Application in the Eastern Tropical Pacific"],"prefix":"10.1002","volume":"16","author":[{"given":"Juan C.","family":"Cubillos\u2010M","sequence":"first","affiliation":[{"name":"Th\u00fcnen Institute of Sea Fisheries  Bremerhaven Germany"},{"name":"Ecological Genomics Group, Institute of Biology and Environmental Sciences University of Oldenburg  Oldenburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annmarie","family":"Fearing","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demian D.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Sharks and Rays Conservation Research Program Mote Marine Laboratory &amp; Aquarium  Sarasota Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Kyne","sequence":"additional","affiliation":[{"name":"Research Institute for the Environment and Livelihoods Charles Darwin University  Darwin Northern Territory Australia"},{"name":"Aurora Biodiversity LLC  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Joana","family":"Dias","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Predator Ecology and Conservation Lab, Biological Sciences Department Florida International University  North Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan N.","family":"Lehman","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaliana","family":"Chichaco","sequence":"additional","affiliation":[{"name":"Smithsonian Tropical Research Institute Smithsonian Institution  Panama City Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan L.","family":"Huerta\u2010Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla L.","family":"McCulloch","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Toepfer","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"},{"name":"Department and Marine and Environmental Sciences Savannah State University  Savannah Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John K.","family":"Carlson","sequence":"additional","affiliation":[{"name":"National Marine Fisheries Service Southeast Fisheries Science Center  Panama City Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4138-4966","authenticated-orcid":false,"given":"Nicole M.","family":"Phillips","sequence":"additional","affiliation":[{"name":"School of Biological, Environmental, and Earth Sciences The University of Southern Mississippi  Hattiesburg Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2018.00133"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2024.1290661"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.115"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2024.108706"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3329\/bjz.v46i2.39057"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.70413"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010018\u20100192\u20106"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aqc.3591"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.18778"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.211045"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201062659\u2010y"},{"key":"e_1_2_11_14_1","unstructured":"Espinoza M. 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