{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:03:19Z","timestamp":1789610599663,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Gastropods rank among the most species\u2010rich animal classes, accounting for 5% (4\u20138) of all described animal species with about 1\/3 of microgastropods (&lt;\u20095\u2009mm in size). DNA extraction from preserved microgastropods retracted into their shells is challenging because established methods usually damage the shell. This study tests the HotSHOT alkaline lysis method as a nondestructive protocol for small ethanol\u2010preserved gastropods. Using a brief lysis step at high pH and temperature, we achieved effective DNA extraction suitable for COI barcode amplification while preserving shell integrity and fragile periostracal structures. High\u2010quality full\u2010length sequences were obtained from 76% of the samples, and 85% of the sequences were suitable for species identification. The remaining samples (15%) did not yield usable sequence data, possibly due to poor preservation, insufficient buffer access in tightly closed shells, or potential PCR problems. Specimens with fully closed opercula showed reduced success unless buffer access was facilitated. Our results show that HotSHOT is a fast and inexpensive method for DNA extraction that preserves shell vouchers, making it ideal for large\u2010scale barcoding of retracted microgastropods and valuable when working with rare specimens. By its low cost and high speed, it has a high potential to accelerate species discovery and delimitation, diversity assessments and monitoring and thus support conservation.<\/jats:p>","DOI":"10.1002\/ece3.74031","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:55:02Z","timestamp":1784123702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preserving Shell Integrity of Microgastropods With the Nondestructive\n                    <scp>HotSHOT DNA<\/scp>\n                    Extraction Method"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9118-9851","authenticated-orcid":false,"given":"Nina","family":"Tombers","sequence":"first","affiliation":[{"name":"Stuttgart State Museum of Natural History  Stuttgart Germany"},{"name":"Vogelwarte, Zoologisches Institut Und Museum Ernst\u2010Moritz\u2010Arndt\u2010Universit\u00e4t Greifswald  Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0140-3859","authenticated-orcid":false,"given":"Cristina","family":"Vasili\u021ba","sequence":"additional","affiliation":[{"name":"Stuttgart State Museum of Natural History  Stuttgart Germany"},{"name":"Karlsruhe Institute of Technology, Institute for Photon Science and Synchrotron Radiation (IPS)  Eggenstein\u2010Leopoldshafen Germany"},{"name":"Center for Biodiversity and Integrative Taxonomy Research, Institute of Biology, University of Hohenheim,  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5432-6987","authenticated-orcid":false,"given":"Ira","family":"Richling","sequence":"additional","affiliation":[{"name":"Stuttgart State Museum of Natural History  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010008\u20101127\u20109"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/psyc\/8887456"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.09.036"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.11646\/zoosymposia.1.1.17"},{"key":"e_1_2_10_7_1","unstructured":"B\u00e1nki O. Y.Roskov M.D\u00f6ring et\u00a0al.2025.\u201cCatalogue of Life. Versions 2025\u201010\u201010 XR.\u201dWith Nicolas Bailly Maria Marta Cigliano Mark John Costello Wim Decock Anne Fuchs Ji Li\u2010Qiang Jeyaraney Kathirithamby et\u00a0al. Catalogue of Life Foundation.https:\/\/doi.org\/10.48580\/DGTPL."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10750\u2010010\u20100094\u20101"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1095\u20108312.2002.00052.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4002\/040.066.0102"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2010.04.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/zoj.12447"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15257770.2025.2486368"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","unstructured":"Feng V. C.H\u00f8egh\u2010Guldberg A.Bu\u010dek andR.Meier.2025.\u201cBalancing Barcoding and Genomics: gDNA Quality in Insect Vouchers After HotSHOT DNA Extraction.\u201dbioRxiv2025\u201310.https:\/\/doi.org\/10.1101\/2025.10.09.680689.","DOI":"10.1101\/2025.10.09.680689"},{"issue":"5","key":"e_1_2_10_15_1","first-page":"294","article-title":"DNA Primers for Amplification of Mitochondrial Cytochrome c Oxidase Subunit I From Diverse Metazoan Invertebrates","volume":"3","author":"Folmer O.","year":"1994","journal-title":"Molecular Marine Biology and Biotechnology"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0281759"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.11646\/zoosymposia.1.1.5"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2144\/btn\u20102023\u20100054"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4003\/006.038.0209"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen\u20102015\u20100172"},{"key":"e_1_2_10_21_1","first-page":"107","article-title":"Two Tiny Streams in Thimphu, With Two New Gastropods (Caenogastropoda, Rissooidea, Pomatiopsidae, Amnicolidae)","volume":"87","author":"Gyeltshen C.","year":"2023","journal-title":"Basteria"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.523.6066"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syac033"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13127\u2010019\u201000400\u20108"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1071.66417"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1022795418060078"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forc.2020.100275"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jzs.12352"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2018.11.027"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095003"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/zsc.12374"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1641\/0006\u20103568(2004)054%5B0321:TGDONM%5D2.0.CO;2"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/biosci\/biaa164"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.031"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112619"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12686\u2010013\u20109893\u20109"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13355-017-0481-4"},{"key":"e_1_2_10_38_1","unstructured":"MolluscaBase.2025.\u201cMolluscaBase.\u201dhttps:\/\/www.molluscabase.org."},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2008.6.218"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.04150\u201013"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009093"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1525\/california\/9780520250925.001.0001"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755\u20100998.2010.2839.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.4003\/006.032.0204"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.13139"},{"issue":"4","key":"e_1_2_10_46_1","first-page":"381","article-title":"An Efficient DNA Extraction Method for Small Metazoans","volume":"6","author":"Schizas N. 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