{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:06:43Z","timestamp":1788923203562,"version":"build-2803163510"},"reference-count":78,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Viola hybanthoides<\/jats:italic>\n                    is a narrowly endemic species restricted to two isolated danxia landscapes (Pingshi and Mount Danxiashan) in Southeast China, serving as an ideal model for studying endemism in fragmented habitats. Using ddRAD\u2010seq and shallow genome sequencing, we genotyped 156 individuals from 13 populations of the species to explore its genetic patterns and evolutionary processes. Results showed extremely low within\u2010population genetic diversity (\n                    <jats:italic>H<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>E<\/jats:italic>\n                    <\/jats:sub>\n                    \u2009=\u20090.058\u2009\u00b1\u20090.028), probably driven by limited pollen and seed dispersal ranges, recurrent founder events, and persistent genetic drift exacerbated by post\u2010glacial population contractions. Pronounced genetic differentiation existed between Pingshi and Danxiashan, with Danxiashan populations showing an east\u2013west split by the Jinjiang River. Admixture analysis and plastid phylogeny identified one population of Pingshi as a hybrid population between Danxiashan and Pingshi. Combined with population dynamics and fastsimcoal2 inference, it is inferred that\n                    <jats:italic>V. hybanthoides<\/jats:italic>\n                    expanded during glacial periods and contracted to refugia in interglacials, with Danxiashan populations spreading northwestward to Pingshi in glaciations, giving rise to secondary contact and hybrid lineages. Thus, the species faces threats including depleted genetic variation, severe habitat fragmentation, and human\u2010exacerbated historical bottlenecks. We propose targeted conservation strategies, including prioritized in\u00a0situ protection of core genetically representative populations, population reinforcement via tissue culture and reintroduction, and long\u2010term multi\u2010indicator population dynamic monitoring. This study elucidates how Quaternary climate fluctuations and danxia topographic isolation jointly shape the genomic differentiation of endemic plants and provides population\u2010genomic references for biodiversity conservation in fragmented edaphic island ecosystems.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74043","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T08:27:34Z","timestamp":1785313654000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quaternary Climate Oscillations Shape Genetic Diversity and Spatial Structure of\n                    <i>Viola hybanthoides<\/i>\n                    (Violaceae) Endemic to Danxia Landscape"],"prefix":"10.1002","volume":"16","author":[{"given":"Sufang","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Stress Biology, School of Life Sciences Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanshuang","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Stress Biology, School of Life Sciences Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangdong Ecological and Environmental Monitoring Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiang","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Danxiashan National Nature Reserve Administration  Shaoguan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Danxiashan National Nature Reserve Administration  Shaoguan 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