{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:06:25Z","timestamp":1788923185932,"version":"build-2803163510"},"reference-count":81,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006788","name":"Great Lakes Fishery Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006788","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The concept of assisted evolution suggests that hurdles to species restoration in modified environments might be overcome by selective breeding of individuals better\u2010suited to the contemporary evolutionary landscape. Here, we introduce an experimental program aimed at developing an Atlantic salmon broodstock resilient to dietary thiamine deficiency complex (TDC). Dietary TDC is caused by overconsumption of lipid\u2010rich prey items containing thiaminase, leading to insufficient maternal allocation of thiamine to eggs and high offspring mortality. Within Lake Champlain, USA, where TDC is widespread due to consumption of invasive Alewife, we conducted offspring survival trials of 114 wild\u2010reared salmon pairs. We documented among\u2010family patterns in offspring survival to yolk\u2010sac absorption. Some females allocated sufficient free thiamine to eggs to avoid severe offspring mortality from TDC, while most did not and suffered complete or severe reproductive failure. Offspring from \u201cresilient\u201d families with high survival were used as founders of a putatively \u201cTDC\u2010Tolerant\u201d broodstock but at the expense of low effective population size (\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                    \u2009=\u200948). We also developed a control broodstock that included additional families that only survived after supplemental thiamine treatment (MAX\u2010Gen, no selection,\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                    \u2009=\u2009152) to represent a status quo scenario. From 2016 to 2025 we (1) developed a Genotyping\u2010in\u2010Thousands by Sequencing (GT\u2010seq) marker panel (2) implemented parentage\u2010based tagging (3) curated two experimental broodstocks and released ~1.6 million of their offspring into Lake Champlain and (4) implemented a sampling program to monitor demographic and evolutionary outcomes. In Lake Champlain, TDC continues to be a major restoration bottleneck afflicting 42%\u201376% of salmon evaluated annually since 2007 and is an increasing conservation issue globally. Continuation of this work will determine whether assisted evolution improved TDC\u2010resistance in the wild and also evaluate the genetic and demographic consequences of contrasting broodstock strategies.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74122","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T02:33:43Z","timestamp":1786156423000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Genetic Monitoring of a Putatively Thiamine Deficiency Complex Tolerant Atlantic Salmon Broodstock"],"prefix":"10.1002","volume":"16","author":[{"given":"Kurt C.","family":"Heim","sequence":"first","affiliation":[{"name":"U.S. Fish and Wildlife Service, Lake Champlain Fish and Wildlife Conservation Office  Essex Junction Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7371-4002","authenticated-orcid":false,"given":"Avril M.","family":"Harder","sequence":"additional","affiliation":[{"name":"College of Forestry, Wildlife and Environment Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie A.","family":"Earley","sequence":"additional","affiliation":[{"name":"U.S. Fish and Wildlife Service, Lake Champlain Fish and Wildlife Conservation Office  Essex Junction Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Boynton","sequence":"additional","affiliation":[{"name":"U.S. Fish and Wildlife Service White River National Fish Hatchery  Bethel Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"Hanlon","sequence":"additional","affiliation":[{"name":"U.S. Fish and Wildlife Service Dwight D. Eisenhower National Fish Hatchery  North Chittenden Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6247-2551","authenticated-orcid":false,"given":"Jacques","family":"Rinchard","sequence":"additional","affiliation":[{"name":"Department of Environmental Science and Ecology State University of New York Brockport  Brockport New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Kelsey","sequence":"additional","affiliation":[{"name":"Vermont Fish and Wildlife Department Ed Weed Fish Culture Station  Grand Isle Vermont USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey A.","family":"Nerkowski","sequence":"additional","affiliation":[{"name":"U.S. Fish and Wildlife Service Northeast Fishery Center  Lamar Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan R.","family":"Campbell","sequence":"additional","affiliation":[{"name":"GTseek LLC  Twin Falls Idaho USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Ardren","sequence":"additional","affiliation":[{"name":"U.S. Fish and Wildlife Service, Lake Champlain Fish and Wildlife Conservation Office  Essex Junction Vermont USA"},{"name":"U.S. Fish and Wildlife Service Silvio O. 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