{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:06:48Z","timestamp":1788923208110,"version":"build-2803163510"},"reference-count":89,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P36167"],"award-info":[{"award-number":["P36167"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Obtaining fresh DNA samples for biodiversity studies is often challenging. Natural history collections offer an alternative way to obtain data, although the DNA from dry\u2010preserved specimens of historical collections is typically degraded and potentially contaminated. Here, we tested three standard DNA isolation methods to obtain DNA for genomic sequencing approaches using 20 identical ethanol and dry\u2010preserved beetle specimens. The best\u2010performing method was applied to a larger set of fresh and historical specimens representing 74 species of the genus\n                    <jats:italic>Trichodes<\/jats:italic>\n                    and 33 species from other genera of Cleridae (Coleoptera) to assess how specimen age and the amount of isolated DNA affect DNA sequence quality measures. Unlike most previous studies, we assessed the quality of isolated DNA through fragment length analysis and whole\u2010genome sequencing. The here used silica\u2010column based oligo isolation protocol for short DNA fragments outperformed standard silica\u2010column based and precipitation\u2010based isolation methods for historical genomic DNA. Fragment length of isolated DNA slightly declined with sample age in dry\u2010preserved specimens, whereas the amount of isolated DNA remained unaffected. Higher DNA amounts led to significantly more recovered loci and longer multiple sequence alignments with fewer gaps. Contamination levels were significantly higher in dry\u2010preserved samples. Our findings demonstrated the suitability of commonly available DNA isolation kits for dry\u2010preserved museum specimens for next generation sequencing without requiring dedicated cleanroom facilities or hazardous reagents. Compared to sample age, the absolute amount of isolated DNA had higher impact on sequence quality and substantial genomic sequence information could be recovered even from old dry\u2010preserved specimens. Our results underscore the potential of historic insect specimens as a source of molecular data with easy to apply DNA isolation protocols.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74137","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:52:27Z","timestamp":1786693947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Quality of Historic\n                    <scp>DNA<\/scp>\n                    From Pinned Beetles"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9571-3481","authenticated-orcid":false,"given":"Daniel","family":"Lukic","sequence":"first","affiliation":[{"name":"Department Environment &amp; Biodiversity Paris Lodron University  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malte","family":"Petersen","sequence":"additional","affiliation":[{"name":"Research IT Department University Hospital Bonn  Bonn Germany"},{"name":"University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeel","family":"Patel","sequence":"additional","affiliation":[{"name":"University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0445-9639","authenticated-orcid":false,"given":"Rama S.","family":"Krovi","sequence":"additional","affiliation":[{"name":"Institute of Systematics and Evolution of Animals Polish Academy of Sciences  Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3524-7153","authenticated-orcid":false,"given":"Dirk","family":"Ahrens","sequence":"additional","affiliation":[{"name":"Museum Alexander Koenig Leibniz Institute for the Analysis of Biodiversity Change  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Gerstmeier","sequence":"additional","affiliation":[{"name":"Zoologische Staatssammlung M\u00fcnchen  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-0640","authenticated-orcid":false,"given":"Jonas","family":"Eberle","sequence":"additional","affiliation":[{"name":"Department Environment &amp; Biodiversity Paris Lodron University  Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198856566.001.0001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045549"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00074"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121139"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2022.893088"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.24678.1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.11646\/megataxa.1.1.10"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/ieaf062"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2020.00094"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020059"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.70078"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv768"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010024\u201000801\u20102"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1570\u20107458.2001.00784.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13072"},{"key":"e_1_2_10_17_1","first-page":"29","volume-title":"Molecular Systematics","author":"Dessauer H. 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