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To bridge this gap, we sequenced and analyzed the complete mitochondrial genome of a specimen from the South China Sea to characterize its structural features, codon usage patterns, and phylogenetic relationships. The 16,569\u2009bp mitogenome (GenBank: PP190474.1) encodes 13 protein\u2010coding genes (PCGs), 22 tRNAs, two rRNAs, and two non\u2010coding regions, exhibiting a distinct A\u2009+\u2009T bias. All tRNAs fold into typical cloverleaf secondary structures except tRNA\u2010Ser\n                    <jats:sup>(AGN)<\/jats:sup>\n                    , which lacks the dihydrouridine (DHU) arm. The control region contains palindromic motifs (TACAT\/ATGTA) capable of forming hairpin structures and five conserved sequence blocks, whereas the O\n                    <jats:sub>L<\/jats:sub>\n                    region harbors a conserved 5\u2032\u2010GCCGG\u20103\u2032 motif. RSCU analysis revealed 31 frequently used codons (RSCU &gt;\u20091) with a pronounced preference for A\/C\u2010ending codons. The \u0394RSCU method identified 10 candidate optimal codons (GCA, CAA, GAA, GGA, AUU, CUA, CCA, CGA, ACA, and GUC). Selection pressure analysis using EasyCodeML and site\u2010specific models indicated that all PCGs are predominantly under purifying selection, with no significant evidence of pervasive positive selection.\n                    <jats:italic>ND6<\/jats:italic>\n                    exhibited elevated pairwise Ka\/Ks ratios (mean\u2009=\u20091.209\u2009\u00b1\u20090.047), consistent with reduced selective constraint rather than adaptive evolution. Phylogenetic analysis of 19 Holocentriformes species using maximum likelihood and Bayesian inference with partitioned models based on 13 PCGs and two rRNA genes (12S and 16S) assigned all taxa to two well\u2010supported subfamilies (Holocentrinae and Myripristinae). Within Holocentrinae,\n                    <jats:italic>Neoniphon<\/jats:italic>\n                    species form a monophyletic clade nested within a paraphyletic\n                    <jats:italic>Sargocentron<\/jats:italic>\n                    , suggesting that the genus\n                    <jats:italic>Sargocentron<\/jats:italic>\n                    as currently defined is not monophyletic. This study provides useful baseline molecular data for further exploration of the evolutionary history of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. argenteus<\/jats:italic>\n                    <\/jats:styled-content>\n                    and other members of Holocentriformes.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74291","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:30:51Z","timestamp":1788514251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights Into the Structural Features, Codon Usage Patterns, and Phylogenetic Analysis in\n                    <i>Neoniphon argenteus<\/i>\n                    (Teleostei: Holocentriformes) Based on Complete Mitochondrial Genome"],"prefix":"10.1002","volume":"16","author":[{"given":"Lei","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) Jinan University  Guangzhou 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D. E. N. K.Iverson J. J.Sterling R. J.Weaver B. A.Maclaine andJ. 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