{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:05:03Z","timestamp":1790726703919,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The complete chloroplast genomes of five\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    cultivars (\u201cYangguang Nanhai,\u201d \u201cYangguang N\u00fchai,\u201d \u201cXu Ri,\u201d \u201cYang Gang,\u201d and \u201cZhao Yang\u201d) were sequenced and analyzed in this study, with publicly available\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    accessions from NCBI included for intraspecific comparative analysis to elucidate cultivar\u2010level genomic variations, genetic diversity and phylogenetic placement. The chloroplast genomes showed typical circular quadripartite structures, ranging from 159,391 to 159,769\u2009bp with GC contents of 35.47%\u201335.56%. Each genome encoded 131\u2013133 genes, including 86\u201389 protein\u2010coding genes, 36\u201337 tRNAs, and 8 rRNAs, with minor intraspecific variations in gene content mainly attributed to tRNA pseudogenization. In total, 154\u2013164 simple sequence repeats (SSRs) and 63\u2013155 long repeats were identified, with SSRs predominantly comprising mononucleotide A\/T repeats; cultivar\u2010specific repeat loci were detected that can serve as candidate markers for cultivar authentication. Nucleotide diversity analysis revealed hypervariable regions (e.g.,\n                    <jats:italic>trnH\u2010GUG_psbA<\/jats:italic>\n                    ,\n                    <jats:italic>ndhF_rpl32<\/jats:italic>\n                    ) at the intraspecific level that could serve as complementary molecular markers for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    germplasm identification. IR boundary analysis revealed minor length fluctuations of boundary genes (e.g.,\n                    <jats:italic>rps19<\/jats:italic>\n                    ,\n                    <jats:italic>ycf1<\/jats:italic>\n                    ) among cultivars, representing intraspecific structural polymorphism rather than major evolutionary events. In phylogenetic analysis, the five cultivars were clustered into a single well\u2010supported clade together with other\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    accessions, providing robust support for the monophyly of the genus\n                    <jats:italic>Ulmus<\/jats:italic>\n                    and confirming the close maternal genetic background of these cultivated materials. Overall, this study presents the first complete chloroplast genome resource for these five\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    cultivars, fills the gap of intraspecific plastome variation for cultivated\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ulmus pumila<\/jats:italic>\n                    <\/jats:styled-content>\n                    germplasm, and establishes a molecular foundation for germplasm identification, maternal lineage tracing and genetic breeding of\n                    <jats:italic>Ulmus<\/jats:italic>\n                    species.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74352","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:20:17Z","timestamp":1790724017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Complete Chloroplast Genomes of Five\n                    <scp>\n                      <i>Ulmus pumila<\/i>\n                    <\/scp>\n                    Cultivars: Comparative Analysis, Evolutionary Dynamics, and Germplasm Authentication Implications"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0733-0969","authenticated-orcid":false,"given":"Ning","family":"Wang","sequence":"first","affiliation":[{"name":"College of Forestry, College of Landscape and Tourism, Key Laboratory of National Forestry and Grassland Administration on Colorful Tree, Hebei Key Laboratory of Floral Biological Breeding Hebei Agricultural University  Baoding 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