{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:49:28Z","timestamp":1791431368516,"version":"4.3.3"},"reference-count":88,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003550","name":"Queensland Government","doi-asserted-by":"publisher","award":["CSAT22032"],"award-info":[{"award-number":["CSAT22032"]}],"id":[{"id":"10.13039\/501100003550","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Following decades of clearing, fragmentation and degradation of coastal heathlands in southeast Queensland, many endemic species are now threatened with extinction, including the vulnerable sand yabby\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cherax robustus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (Parastacidae), a freshwater crayfish restricted to 200km\n                    <jats:sup>2<\/jats:sup>\n                    of acidic wetlands. Dramatic fluctuations in the distribution and extent of these wetlands, particularly during the glacial cycles of the Pleistocene, likely shaped the distribution and genetic structure of acid\u2010specialist species, yet their historic and contemporary connectivity remains poorly understood, despite these species being particularly sensitive to habitat fragmentation and environmental change. Here, we investigate the genetic structure of two island and two mainland\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. robustus<\/jats:italic>\n                    <\/jats:styled-content>\n                    populations within peatlands using DArTseq next\u2010generation DNA sequencing. Analysis of over 7000 SNP markers indicates population divergence occurred during the mid\u2010to\u2010late Pleistocene, with no detectable gene flow between most sites for between 50,000 and 120,000\u2009years. Consistent with long\u2010term isolation, we found slight heterozygote deficiency in all populations, and evidence of genetic erosion in only one population, which should continue to be monitored, though the effect appears to predate European arrival in Australia. Evidently, sand yabbies are not naturally rare within stable environments but have become vulnerable through anthropogenic pressures of habitat loss and degradation. The strong population structuring observed means that each population represents a unique genetic lineage and should be managed as an independent unit. Protecting all remaining habitat and preventing any further degradation is therefore critical to the conservation of this species as well as the wider ecosystem.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74364","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:06:06Z","timestamp":1789693566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pleistocene Legacies in the Contemporary Population Genetic Structure of an Acid\u2010Specialised Crayfish, the Threatened Sand Yabby\n                    <scp>\n                      <i>Cherax robustus<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5511-0490","authenticated-orcid":false,"given":"Grace","family":"Smith","sequence":"first","affiliation":[{"name":"Centre for Bioinnovation, School of Science Technology and Engineering University of the Sunshine Coast  Sippy Downs Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0777-2367","authenticated-orcid":false,"given":"Tomer","family":"Ventura","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, School of Science Technology and Engineering University of the Sunshine Coast  Sippy Downs Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Yule","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, School of Science Technology and Engineering University of the Sunshine Coast  Sippy Downs Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2005.0353"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12559"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1739.1995.09030686.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38626-3"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1651\/C-2518"},{"key":"e_1_2_11_7_1","unstructured":"Bentley A. I.2007.\u201cPhylogeographic Structure of Freshwater Crayfish of the GenusCherax(Decapoda: Parastacidae) on the Mainland and Islands of Southeast Queensland. Honours thesis. Griffith School of Environment Griffith University.\u201d"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1127\/1863-9135\/2008\/0173-0153"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-4571.2011.00214.x"},{"key":"e_1_2_11_10_1","unstructured":"Boyd R. K.Ruming S.Davies T.Payenberg andS.Lang.2004.\u201cFraser Island and Hervey Bay\u2010a Classic Modern Sedimentary Environment.\u201dPetroleum Exploration Society of Australia (PESA) Eastern Australasian Basins Symposium II. Adelaide."},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margeo.2008.02.013"},{"issue":"2","key":"e_1_2_11_12_1","first-page":"175","article-title":"A Review of Research Findings Concerned With Pastoral Development on the Wallum of South\u2010Eastern Queensland","volume":"7","author":"Bryan W.","year":"1973","journal-title":"Tropical Grasslands"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0025-3227(92)90173-F"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.quascirev.2008.08.032"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1201\/b21842"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0025-3227(86)90036-8"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geodrs.2024.e00913"},{"key":"e_1_2_11_18_1","volume-title":"The Ecosystem of the Coastal Lowlands (Wallum) of Southern Queensland","author":"Coaldrake J. E.","year":"1961"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89056-z"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3310"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.9837"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1071\/MF25063"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34811-y"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41561-022-01062-6"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab468"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-023-01572-9"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.13204"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1739.1998.96456.x"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2012.04.034"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12382"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.563885"},{"key":"e_1_2_11_32_1","doi-asserted-by":"crossref","unstructured":"Garnier\u2010G\u00e9r\u00e9 P. andL.Chikhi.2013.\u201cPopulation Subdivision Hardy\u2013Weinberg Equilibrium and the Wahlund Effect.\u201dEncyclopedia of Life Sciences.","DOI":"10.1002\/9780470015902.a0005446.pub3"},{"key":"e_1_2_11_33_1","unstructured":"Garvie L.1998.\u201cPopulation Genetic Structure and Conservation Status of an Endemic Habitat\u2010Specialist Macroinvertebrate (Cherax robustus) in South\u2010East Queensland. Honours thesis Queensland University of Technology.\u201d"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2004.00828.x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12745"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1739.2006.00530.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/09596836221126120"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/35016000"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/fwb.12099"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2540.1999.00539.x"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.17411"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-870-2_5"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3354\/esr00414"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.5962\/p.357745"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.palaeo.2015.10.033"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.quascirev.2012.09.006"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gecco.2022.e02301"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1071\/WF12128"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.5962\/p.357757"},{"issue":"3","key":"e_1_2_11_50_1","first-page":"140","article-title":"Land Development for Beef Production in the Wallum","volume":"21","author":"McGuire K.","year":"1968","journal-title":"Quarterly Review of the Rural Economy"},{"key":"e_1_2_11_51_1","unstructured":"Meyer E.2006.\u201cNational Recovery Plan for the Wallum Sedgefrog and Other Wallum\u2010Dependent Frog Species Queensland. Parks and Wildlife Service\u201d."},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.13918"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12995"},{"key":"e_1_2_11_54_1","unstructured":"Morgan D. andS.Beatty.2005.Fish and crayfish fauna of Ellen Brook Cowaramup Brook and Gunyulgup Brook in the Cape to Cape Region of Western Australia."},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-5347(94)90057-4"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1071\/MF14359"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/jzo.12466"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41437-017-0037-y"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.04874"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13951"},{"key":"e_1_2_11_61_1","volume-title":"Nomination to Change the Conservation Class of Cherax robustus Under the Queensland Nature Conservation Act 1992","author":"Page T. J.","year":"2021"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800932"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2007.52.3.1055"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2427.2011.02713.x"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1071\/MF03139"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp696"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty633"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1080\/08120099.2018.1558417"},{"key":"e_1_2_11_69_1","doi-asserted-by":"crossref","unstructured":"Payenberg T. H. R.Boyd J.Beaudoin et\u00a0al.2006.The Filling of An Incised Valley By Shelf Dunes\u2014An Example From Hervey Bay East Coast of Australia.","DOI":"10.2110\/pec.06.85.0087"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/d14060436"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/aec.13504"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2005.3425"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12228"},{"key":"e_1_2_11_74_1","unstructured":"Renwick J.2006.\u201cPopulation Structure and Genetic Diversity of Southeast Queensland Populations of the Wallum Froglet Crinia tinnula(Tschudi). PhD Thesis. Queensland University of Technology.\u201d"},{"issue":"4","key":"e_1_2_11_75_1","first-page":"406","article-title":"The Neighbor\u2010Joining Method: A New Method for Reconstructing Phylogenetic Trees","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Molecular Biology and Evolution"},{"key":"e_1_2_11_76_1","volume-title":"The Conservation Status of Queensland's Bioregional Ecosystems","author":"Sattler P. S.","year":"1999"},{"key":"e_1_2_11_77_1","unstructured":"Scully O.2024.\u201cDietary Studies of the Acid\u2010Specialist CrayfishCherax Robustus.\u201dUndergraduate Thesis University of the Sunshine Coast."},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1071\/MF07235"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2010.02843.x"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/ecs2.1384"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.quascirev.2024.108529"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043292"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/jqs.2981"},{"key":"e_1_2_11_84_1","unstructured":"UNEP.2022.\u201cGlobal Peatlands Assessment: The State of the World's Peatlands \u2010 Evidence for Action Toward the Conservation Restoration and Sustainable Management of Peatlands.\u201d"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1111\/eva.70142"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1002\/brv.70135"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1984.tb05657.x"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.1071\/MF99011"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1965.tb01731.x"}],"container-title":["Ecology and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.74364","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ece3.74364","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ece3.74364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:51:48Z","timestamp":1791427908000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ece3.74364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":88,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/ece3.74364"],"URL":"https:\/\/doi.org\/10.1002\/ece3.74364","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.22541\/authorea.15003163\/v1","asserted-by":"object"}]},"ISSN":["2045-7758","2045-7758"],"issn-type":[{"value":"2045-7758","type":"print"},{"value":"2045-7758","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e74364"}}