{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:34:02Z","timestamp":1793140442396,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013948","name":"Tempus K\u00f6zalap\u00edtv\u00e1ny","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013948","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ecology and Evolution"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Coat color is an important visible phenotype in sheep and may reflect underlying differences in pigmentation\u2010related molecular regulation. However, the transcriptomic mechanisms underlying black and white coat\u2010color divergence in Hortob\u00e1gyi Racka sheep remain insufficiently characterized. To address this, this study employed high\u2010throughput RNA sequencing to analyze the skin transcriptome profiles of black\u2010 and white\u2010coated Hortob\u00e1gyi Racka sheep and identified functional pathways associated with coat\u2010color difference. We identified 108 DEGs; 25 downregulated and 83 upregulated (FC &gt;\u20091.5,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in black\u2010coated sheep compared with white\u2010coated sheep, with the most significant genes including\n                    <jats:italic>TYRP1, PMEL, TRPM1, MLANA, SLC24A4, SLC45A2, DCT, OCA2,<\/jats:italic>\n                    and\n                    <jats:italic>DDC<\/jats:italic>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    adj &lt;\u20090.05). Overall, the GO, KEGG, and PPI analyses consistently indicated that coat\u2010color divergence in Hortob\u00e1gyi Racka sheep was primarily associated with melanogenesis, melanosome biology, and tyrosine metabolism, while also suggesting possible links with broader cellular process related to redox balance, energy metabolism, and stress responses. However, these broader adaptive interpretations should be considered hypothesis\u2010generating and require further validation using larger cohorts, phenotypic measurements, and functional assays. Our study provides a transcriptomic framework connecting coat\u2010color phenotypes to pigmentation regulatory networks and provides a foundation for investigating the possible adaptive significance of coat\u2010color variation in sheep.\n                  <\/jats:p>","DOI":"10.1002\/ece3.74397","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:31:35Z","timestamp":1791430295000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coat Color\u2010Associated Transcriptomic Differences of Black and White Hortob\u00e1gyi Racka Sheep Using the RNA\u2010Seq Technique"],"prefix":"10.1002","volume":"16","author":[{"given":"Putri Kusuma","family":"Astuti","sequence":"first","affiliation":[{"name":"Centre for Agricultural Genomics and Biotechnology University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Bagi","sequence":"additional","affiliation":[{"name":"Centre for Agricultural Genomics and Biotechnology University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilla","family":"Bodrogi","sequence":"additional","affiliation":[{"name":"Agribiotechnology and Precision Breeding for Food Security National Laboratory, Department of Animal Biotechnology, Institute of Genetics and Biotechnology Hungarian University of Agriculture and Life Sciences  G\u00f6d\u00f6ll\u0151 Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5441-5303","authenticated-orcid":false,"given":"Szilvia","family":"Kusza","sequence":"additional","affiliation":[{"name":"Centre for Agricultural Genomics and Biotechnology University of Debrecen  Debrecen 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